Alanine aminotransferase

Diagnostic Kits used for analysis of serum levels of glucose, triacylglycerol (TA), total cholesterol (TC), high density lipoprotein (HDL) and ketone bodies, activities of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST), the concentration of malondialdehyde (MDA) and the activity of glutathione reductase (GR) were purchased from Bio-diagnostic Company, Cairo, Egypt. Diagnostic Kits used for Enzyme Linked Immune Sorbent Assay (ELISA) for determination of serum leptin and adiponectin levels were purchased from Nova Chemicals Company, Cairo, Egypt. Diagnostic Kits for analysis of UCP-1 protein expression in brown adipose tissue were obtained from Santa Cruz Biotechnology, Inc., Dallas, TX, USA.

Aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), total protein, albumin, creatinine, urea, and uric acids were purchased from Biodiagnostics Company (Dokki, Giza, Egypt). The RNA extraction Kit was purchased from Introbio Company, Cat.No. 17063. Reverse transcriptase Polymerase chain reaction (RT-PCR) sensiFAST™ cDNA synthesis kit (Bioline, United Kingdom). All used primers are commercially based. Aflatoxin from the Mycology and Mycotoxins Department, Animal Health Research Institute (ARC), Dokki, Egypt. Curcumin Nanoparticles (NT-Cur-NPs) were purchased from Nanotech Egypt for photo- electronics at the city of 6 October, Al Giza. Saccharomyces cerevisiae cell wall extract (100%, Beta-glucan: 20% minimum &Manan Oligosaccharides: 20% minimum.) was obtained from IBEX Group for animal nutrition and health products and produced by Shandong Bio Sunkeen Co., Ltd (a state-owned high-tech enterprise that integrates R&D, production and sales with rich experience of yeast and nature yeast products.

TQ was purchased from Sigma-Aldrich Corp. (MO, USA), MTX by Minapharm Pharmaceuticals (Egypt), and TNF-α enzyme-linked immunosorbent assay (ELISA) kit by Wkea Med Supplies Corp. (China). Kits for examining blood urea nitrogen (BUN), creatinine, alanine aminotransferase (ALT), aspartate aminotransferase (AST), reduced glutathione (GSH), and catalase were purchased from Biodiagnostic (Egypt). The ready to use inducible nitric oxide synthase (iNOS), NF-κB/p65, COX-2, and caspase 3 rabbit polyclonal antibodies were purchased from Thermo Fisher Scientific Inc./Lab Vision (CA, USA).