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Agilent 8800 icp triple quad

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The Agilent 8800 ICP Triple Quad is an inductively coupled plasma mass spectrometer (ICP-MS) designed for high-performance trace element analysis. It features a triple quadrupole configuration, allowing for advanced spectral interference removal and precise determination of a wide range of elements at ultra-trace levels.

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Agilent 8800 ICP Triple Quad (ICP-QQQ) instrument Agilent 8800 ICP-MS Triple Quad Agilent 8800 ICP Triple Quad (ICP-QQQ) Agilent 8800

6 protocols using agilent 8800 icp triple quad

1

Maternal-Fetal Lead Exposure Evaluation

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Maternal blood was collected at the 2nd and 3rd trimester visit. Cord blood lead was also collected after parturition. All blood specimens were drawn in trace metal free tubes and refrigerated at 2–6°C until analysis. Lead concentration was measured by external calibration using the Agilent 8800 ICP Triple Quad (Agilent, Santa Clara, CA) in MS/MS mode in the trace metals laboratory at the Icahn School of Medicine at Mount Sinai. The limit of detection was <0.2μg/dL and the instrument precision (given as %RSD) was approximately 5%. Blinded quality control samples obtained from the Maternal and Child Health Bureau and the Wisconsin State Laboratory of Hygiene Cooperative Blood Lead Proficiency Testing Program showed good precision and accuracy.
Saylor C., Tamayo-Ortiz M., Pantic I., Amarasiriwardena C., McRae N., Estrada-Gutierrez G., Parra-Hernandez S., Tolentino M., Baccarelli A.A., Fadrowski J.J., Gennings C., Satlin L.M., Wright R.O., Tellez-Rojo M.M, & Sanders A.P. (2021). Prenatal blood lead levels and reduced preadolescent glomerular filtration rate: Modification by body mass index. Environment international, 154, 106414.
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2

Maternal-Cord Blood Manganese Levels

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Maternal blood was collected by trained research staff at the 2nd trimester visit (12 – 34 weeks; n = 894), 3rd trimester visit (26 – 38 weeks; n = 745), and at birth (maternal n = 732; cord n = 515) and stored at −20°C. Mn was analyzed by inductively coupled plasma-mass spectrometry (ICP-MS/MS) as previously described for Pb with the appropriate quality controls (20 (link)). In brief, blood was digested in HNO3 and 30% H2O2 and analyzed via ICP-MS/MS on an Agilent 8800 ICP Triple Quad (ICP-QQQ) instrument (Agilent technologies, Inc., Delaware, USA) with Indium as the internal standard. The quantitation limit of detection (LOD) was 0.02 – 0.08 μg/dL with no values below the LOD.
Kupsco A., Estrada-Gutierrez G., Cantoral A., Schnaas L., Pantic I., Amarasiriwardena C., Svensson K., Bellinger D.C., Téllez-Rojo M.M., Baccarelli A.A, & Wright R.O. (2020). Modification of the effects of prenatal manganese exposure on child neurodevelopment by maternal anemia and iron deficiency. Pediatric research, 88(2), 325-333.
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3

Maternal-Cord Blood Manganese Levels

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Maternal blood was collected by trained research staff at the 2nd trimester visit (12 – 34 weeks; n = 894), 3rd trimester visit (26 – 38 weeks; n = 745), and at birth (maternal n = 732; cord n = 515) and stored at −20°C. Mn was analyzed by inductively coupled plasma-mass spectrometry (ICP-MS/MS) as previously described for Pb with the appropriate quality controls (20 (link)). In brief, blood was digested in HNO3 and 30% H2O2 and analyzed via ICP-MS/MS on an Agilent 8800 ICP Triple Quad (ICP-QQQ) instrument (Agilent technologies, Inc., Delaware, USA) with Indium as the internal standard. The quantitation limit of detection (LOD) was 0.02 – 0.08 μg/dL with no values below the LOD.
Kupsco A., Estrada-Gutierrez G., Cantoral A., Schnaas L., Pantic I., Amarasiriwardena C., Svensson K., Bellinger D.C., Téllez-Rojo M.M., Baccarelli A.A, & Wright R.O. (2020). Modification of the effects of prenatal manganese exposure on child neurodevelopment by maternal anemia and iron deficiency. Pediatric research, 88(2), 325-333.
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4

Maternal blood lead analysis protocol

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Maternal venous whole blood samples were collected at the second trimester visit. Blood samples were drawn in trace metal free tubes and stored at 2–6 °C until analysis. Samples were prepared and analyzed for lead levels using the Agilent 8800 ICP Triple Quad (ICP-QQQ) in MS/MS mode at the Lautenberg Environmental Health Sciences Laboratory at the Icahn School of Medicine at Mount Sinai in New York (Renzetti et al., 2017 (link)). The limit of detection was 0.02 μg/dL; only one sample was below the LOD and was imputed to 0.02 divided by 2 .
Sanders A.P., Svensson K., Gennings C., Burris H.H., Oken E., Amarasiriwardena C., Basnet P., Pizano-Zarate M.L., Schnaas L., Tamayo-Ortiz M., Baccarelli A.A., Satlin L.M., Wright R.O, & Tellez-Rojo M.M. (2018). Prenatal lead exposure modifies the effect of shorter gestation on increased blood pressure in children. Environment international, 120, 464-471.
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5

Maternal blood lead analysis protocol

Cited 1 time
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Maternal venous whole blood samples were collected at the second trimester visit. Blood samples were drawn in trace metal free tubes and stored at 2–6 °C until analysis. Samples were prepared and analyzed for lead levels using the Agilent 8800 ICP Triple Quad (ICP-QQQ) in MS/MS mode at the Lautenberg Environmental Health Sciences Laboratory at the Icahn School of Medicine at Mount Sinai in New York (Renzetti et al., 2017 (link)). The limit of detection was 0.02 μg/dL; only one sample was below the LOD and was imputed to 0.02 divided by 2 .
Sanders A.P., Svensson K., Gennings C., Burris H.H., Oken E., Amarasiriwardena C., Basnet P., Pizano-Zarate M.L., Schnaas L., Tamayo-Ortiz M., Baccarelli A.A., Satlin L.M., Wright R.O, & Tellez-Rojo M.M. (2018). Prenatal lead exposure modifies the effect of shorter gestation on increased blood pressure in children. Environment international, 120, 464-471.
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6

Trace Lead Levels in Children's Blood

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Children's venous blood was collected at the 4-year visit. All blood specimens were drawn in trace metal free tubes, refrigerated at 4°C until shipment to the laboratory where they were frozen at -20°C until analysis. Lead concentration was measured by external calibration using the Agilent 8800 ICP Triple Quad (Agilent, Santa Clara, CA) in MS/MS mode in the trace metals laboratory at the Icahn School of Medicine at Mount Sinai. The limit of detection was <0.2 μg/dL and the instrument precision (given as %RSD) was approximately 5%. Blinded quality control samples obtained from the Maternal and Child Health Bureau and the Wisconsin State Laboratory of Hygiene Cooperative Blood Lead Proficiency Testing Program showed good precision and accuracy.
Quality control (QC) was implemented by analyzing and verifying the initial calibration; continuing calibration verification standards; NIST traceable mixed-element standard solution at two concentration levels, and procedural blanks. 2% of samples were also subject to repeat analysis.
Saylor C., Malin A.J., Tamayo-Ortiz M., Cantoral A., Amarasiriwardena C., Estrada-Gutierrez G., Tolentino M.C., Pantic I., Wright R.O., Tellez-Rojo M.M, & Sanders A.P. (2022). Early childhood fluoride exposure and preadolescent kidney function. Environmental research, 204(Pt A).
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