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Fitc dextran

Manufactured by Santa Cruz Biotechnology
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FITC-dextran is a fluorescently labeled polysaccharide used as a research tool in various applications. It consists of dextran molecules covalently linked to fluorescein isothiocyanate (FITC), a green fluorescent dye. FITC-dextran is primarily used for tracing and visualization purposes in biological research.

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Lab products found in correlation

High concentrated matrigel, by BD (2 mentions) Fluosphere, by Thermo Fisher Scientific (1 mentions) Bz x800, by Keyence (1 mentions)

Close spelling variants of this product

FITC-dextran and chloroquine

5 protocols using fitc dextran

1

Angiogenesis Assay Using Matrigel

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High-concentrated matrigel (500 µL, BD Biosciences) containing LECs or HUVECs (2×106 per gel) and heparin (10 units per gel) was injected subcutaneously on the ventral side of both flanks of a nude mouse. TCM or SFM (50 µL per injection) was subcutaneously administered daily for 10 days, the mice were euthanized, and the gel plugs were excised and analyzed. For visualizing blood vessels, FITC-dextran (70 kDa, 80 mg kg−1, Santa-Cruz) was injected through the tail-vein 1h before sacrifice.
Lee E., Fertig E.J., Jin K., Sukumar S., Pandey N.B, & Popel A.S. (2014). Breast cancer cells condition lymphatic endothelial cells within pre-metastatic niches to promote metastasis. Nature communications, 5, 4715.
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2

Quantitative Imaging of Vascular Perfusion

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Samples were imaged using Keyence BZ-x800, using a z-stack to capture the entire height of the sample. Max intensity projection of regions of interest was utilized for quantification. Using AutoTube (ETH Zürich), the key parameters of the vessels were quantified. Perfusability and permeability were investigated by introducing 1 μm FluoSpheres (Thermo) or 70 kDa FITC-dextran (Santa Cruz) to the medium channels while taking a timelapse.
Wang E., Andrade M.J, & Smith Q. (2023). Vascularized liver-on-a-chip model to investigate nicotine-induced dysfunction. Biomicrofluidics, 17(6), 064108.
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3

Angiogenesis Assay Using Matrigel

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High-concentrated matrigel (500 µL, BD Biosciences) containing LECs or HUVECs (2×106 per gel) and heparin (10 units per gel) was injected subcutaneously on the ventral side of both flanks of a nude mouse. TCM or SFM (50 µL per injection) was subcutaneously administered daily for 10 days, the mice were euthanized, and the gel plugs were excised and analyzed. For visualizing blood vessels, FITC-dextran (70 kDa, 80 mg kg−1, Santa-Cruz) was injected through the tail-vein 1h before sacrifice.
Lee E., Fertig E.J., Jin K., Sukumar S., Pandey N.B, & Popel A.S. (2014). Breast cancer cells condition lymphatic endothelial cells within pre-metastatic niches to promote metastasis. Nature communications, 5, 4715.
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4

Quantifying Macrophage Endocytosis Capacity

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M0, M1, M2, and BD or HC serum-treated HMDMs were incubated in PBS with 1% BSA at 4°C for 30 min. After washing in PBS, cells were incubated with FITC-Dextran (Santa Cruz) and shaken at room temperature for 30 min. Cells were harvested and washed twice with PBS, 1% BSA. Intracellular FITC-Dextran was determined by flow cytometry.
Wu X., Wang Z., Shi J., Yu X., Li C., Liu J., Zhang F., Chen H, & Zheng W. (2022). Macrophage polarization toward M1 phenotype through NF-κB signaling in patients with Behçet’s disease. Arthritis Research & Therapy, 24, 249.
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5

Transwell Permeability Assay for ECs

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Treated ECs were seeded on transwell inserts with 0.4 μm pore membrane (Costar, 3413) in a 24-well plate, and cultured overnight to reach confluence. Cells were incubated with ECM without phenol red. FITC-dextran (MW = 70,000 Da, Santa Cruz, SC-263323, 10 μg/ml) was added to the top chamber. The medium from the lower chamber was collected, and fluorescence was measured with excitation wavelength at 485 nm and emission at 530 nm.
Huang M., Yang F., Zhang D., Lin M., Duan H., El-Mayta R., Zhang L., Qin L., Shewale S.V., Pei L., Mitchell M.J., Rader D.J., Fan Y, & Gong Y. (2022). Endothelial plasticity drives aberrant vascularization and impedes cardiac repair after myocardial infarction. Nature cardiovascular research, 1(4), 372-388.
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