STED microscopy was performed on a Leica TCS SP8 STED 3X confocal laser-scanning microscope equipped with a HC PL APO CS2 100x/1.40 OIL objective. Confocal sections were imaged with Leica LAS X Core software and processed with Huygens Software Suite (SVI). Images were recorded using 405 nm laser line at 1.4% laser power to image DAPI, and a 572 nm Laser line at 5.6% laser power to image Tubulin in confocal detector mode. LEM2 was imaged with a 653 nm laser line at 2.5% laser power in STED pulsed detector mode (gate start at 0.3 ns and gate end at 6.5 ns) with a Huygens saturation factor of 5.7. Deconvolved Images were contrast enhanced in ImageJ (NIH), raw data available upon request.
Software suite
Manufactured by Leica
The Leica Software Suite is a comprehensive set of applications designed to support various aspects of laboratory workflows. This suite includes tools for data analysis, visualization, and workflow management, aiming to enhance productivity and efficiency in the laboratory environment.
Automatically generated - may contain errors
2 protocols using software suite
1
High-resolution STED Imaging of Cellular Structures
2
High-resolution STED Imaging of Cellular Structures
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