Anti tubulin sc 58886

Manufactured by Santa Cruz Biotechnology

Sourced in United Kingdom

Anti-Tubulin (sc-58886) is a primary antibody product used for the detection and analysis of tubulin proteins in various biological samples. Tubulin is a key structural component of the cytoskeleton and is essential for various cellular processes. This antibody can be utilized in techniques such as Western blotting, immunohistochemistry, and immunocytochemistry to identify and study tubulin expression and localization.

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Lab products found in correlation

Anti erk1 2 137f5, by Cell Signaling Technology (1 mentions) Anti raf b f 7, by Santa Cruz Biotechnology (1 mentions) Goat anti rabbit igg horseradish peroxidase hrp, by Santa Cruz Biotechnology (1 mentions) Anti phospho akt ser473 d9e, by Cell Signaling Technology (1 mentions) 5 fluorouracil 5 fu, by Merck Group (1 mentions) Human fc blocking reagent, by Miltenyi Biotec (1 mentions) Anti akt pan c67e7, by Cell Signaling Technology (1 mentions) Caffeine, by Merck Group (1 mentions) Anti gapdh 14c10, by Cell Signaling Technology (1 mentions) Gemcitabine, by Eli Lilly (1 mentions)

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Anti-tubulin (154 citations)

2 protocols using anti tubulin sc 58886

Plasmid Construction and Antibody Characterization

Cited 1 time

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The plasmids made in this study are listed as follows: pCSIIneo-MCS (multi-cloning site), pCSIIneo-FLAG-BRAF-V600E, pCSIIneo-FLAG-KRAS-G12V, psPAX2 was a gift from Dr. D. Trono, Addgene plasmid #12260) [23 (link)]. pCMV-VSV-G-RSV-Rev (a gift of Dr. Miyoshi, RIKEN, Japan). pCSIIbsr-FLAG-BRAF-V600E was a kind gift from Dr. Matsuda (Kyoto University) [24 (link)].
The antibodies used for western blot and immunofluorescence analyses are as follows: phospho-anti-Erk1/2 (Thr202/Tyr204) (E10) and anti-Erk1/2 (137F5) were from Cell Signaling Technology; anti-KRAS (clone 3B10-2F2) was from Sigma; anti-Raf-B (F-7) and anti-Tubulin (sc-58886) were from Santa Cruz Biotechnology.
The negative control siPOOL, BRAF targeted siPOOL and KRAS targeted siPOOL were purchased from siTOOLs Biotech.

Ito R.E., Oneyama C, & Aoki K. (2021). Oncogenic mutation or overexpression of oncogenic KRAS or BRAF is not sufficient to confer oncogene addiction. PLoS ONE, 16(4), e0249388.

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Taste Receptor Expression Analysis

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Caffeine (Sigma Aldrich, St. Louis, MO, USA) was dissolved in distilled water, Gemcitabine (Eli Lilly & Co., Indianapolis, IN, USA) in phosphate buffered saline (PBS; Sigma Aldrich), and 5-Fluorouracil (5-FU; Sigma Aldrich) in Dimethylsulfoxide (DMSO; Sigma Aldrich). For Western blot analysis the following antibodies were used: anti-ABCG2 (EPR2099, abcam); anti-Phospho-Akt Ser473 (D9E, Cell Signaling, Cambridge, UK), anti-pan-Akt (C67E7, Cell Signaling); anti-GAPDH (14C10, Cell signaling); anti-T2R10 (orb 164582; Biorbyt, Cambridge, UK); anti-tubulin (sc-58886, Santa Cruz); goat anti-rabbit IgG-horse radish peroxidase (HRP; Santa Cruz) as secondary antibody. For flow cytometry, a Fc Blocking Reagent (human, Miltenyi Biotec, Bergisch-Gladbach, Germany) was used; anti-T2R10 (ab138285, abcam, Cambridge, UK), a rabbit polyclonal isotype control (abcam), and anti-rabbit-IgG conjugated with phycoerythrin (PE) (Jackson Immunoresearch, West Grove, PA, USA).

Stern L., Giese N., Hackert T., Strobel O., Schirmacher P., Felix K, & Gaida M.M. (2018). Overcoming chemoresistance in pancreatic cancer cells: role of the bitter taste receptor T2R10. Journal of Cancer, 9(4), 711-725.

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