Frozen fecal samples were vortexed with PBS to obtain a homogenous suspension. Samples were then centrifuged for 10 min at 9,500 g, at 4°C. Supernatants were collected and stored at −80°C until analysis. Lipocalin-2 levels were estimated in the supernatants using Duoset murine lipocalin-2 ELISA kit (R&D Systems, Minneapolis, MN). Calprotectin levels were estimated in the supernatants using the S100A9/Calprotectin mouse ELISA kit (Hycult Biotech, Plymouth Meeting, PA).37 Both were standardized to total protein, as determined using measured with the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA).
Duoset murine lipocalin 2 elisa kit
Manufactured by R&D Systems
The Duoset murine lipocalin-2 ELISA kit is a sandwich enzyme-linked immunosorbent assay (ELISA) designed for the quantitative measurement of mouse lipocalin-2 levels in cell culture supernatants, serum, and plasma samples.
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Lab products found in correlation
3 protocols using duoset murine lipocalin 2 elisa kit
1
Analyzing Fecal Biomarkers: Lipocalin-2 and Calprotectin
2
Analyzing Fecal Biomarkers: Lipocalin-2 and Calprotectin
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Frozen fecal samples were vortexed with PBS to obtain a homogenous suspension. Samples were then centrifuged for 10 min at 9,500 g, at 4°C. Supernatants were collected and stored at −80°C until analysis. Lipocalin-2 levels were estimated in the supernatants using Duoset murine lipocalin-2 ELISA kit (R&D Systems, Minneapolis, MN). Calprotectin levels were estimated in the supernatants using the S100A9/Calprotectin mouse ELISA kit (Hycult Biotech, Plymouth Meeting, PA).37 Both were standardized to total protein, as determined using measured with the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA).
3
Fecal Lipocalin-2 and Albumin Quantification
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Frozen fecal pellets were reconstituted in PBS containing 0.1% Tween 20 (100 mg/mL) and vortexed for 20 min. Fecal homogenates were then centrifuged for 10 min at 10,000g and 4°C, and cleared supernatants were stored at −20°C and further processed through ELISA (Duoset Murine Lipocalin-2 ELISA kit from R&D Systems, Minneapolis, MN; and albumin from Bethyl Laboratories, Montgomery, AL).
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