Anti hsp70 w27

Plasmids encoding mCherry or mCherry C-terminally fused to wild-type (WT) or mutant (∆ALIX and K215A) mouse syntenins, and mCherry-CD63 were as reported before^{23 (link),34 (link),44 (link)}. For syntenin gain-of-function in MEFs, a cDNA encoding full-length non-tagged wild-type mouse syntenin and enhanced green fluorescent protein (Synt IRES eGFP) was cloned in bicistronic pMSCV viral vectors. Luciferase and eGFP (LUC IRES eGFP) were used as control. Open reading frames were confirmed by sequencing. Mouse monoclonal antibodies against the intracellular domain (ICD) of syndecan 1/3 (2E9) and syndecan 2 (6G12), and against remnant desaturated (delta) HS after heparitinase (lyase) digestion (3G10) and native HS (10E4), and rabbit polyclonal antibodies against ALIX (Rb67) and mouse syntenin (Rb96) were all described before^{23 (link),71 (link)–73 (link)}. Other antibodies were from commercial sources; antibodies against mouse CD63 (R5G2) was from MBL; antibodies against syndecan 4 ICD (Abnova, PAB9045), α-tubulin was from (Sigma-Aldrich), against β-actin (AC-15), EGFR (Cell signaling 2232), β1 integrin (BD Pharmingen 553715), fibronectin (BD Transduction 610077 Clone 10), CD81 (D4), anti TSG 101 (C20) and anti-HSP70 (W27) and anti GFP were purchased from Santa Cruz.

Anti-human TRPM1 (F-3, western blotting 1: 250; immunoprecipitation 1:50; immunohistochemistry 1:100), anti-HSP70 (W27, for western blotting, 1:1000), anti-HSP90 α/β (F-8, for western blotting, 1:1000), anti-CDC37 (C-11, for western blotting, 1:1000) antibodies, and anti-HSP90 α/β conjugated agarose were from Santa Cruz Biotechnology. Anti-mouse Trpm1 (western blotting 1:200; immunohistochemistry 1:100; immunofluorescence 1:100) was from Novus Biologicals. Anti-cleaved caspase 3 (Asp175) (western blotting 1:1000; immunohistochemistry 1:100), anti-AKT (western blotting 1:1000; immunohistochemistry 1:100), anti-phospho-AKT(Ser473) (D9E, immunohistochemistry 1:100), anti-β-Actin (13E5, western blotting 1:2000; immunofluorescence 1:500), anti-PARP1 (# 9542, western blotting 1:1000) and anti-Ki67 (D2H10, immunohistochemistry 1:100) antibodies were purchased from Cell Signaling Technology. Anti-Rhodopsin (Rho 1D4, immunohistochemistry 1:100), anti-RPE65 (immunofluorescence 1:250), anti-FLAG M2 (western blotting 1:1000) antibodies and anti-FLAG M2 affinity agarose gel were from Sigma-Aldrich. Anti-GFAP antibody (immunohistochemistry 1:100) was from Dako. Biotinylated Peanut Agglutinin ((immunohistochemistry 1:500) was obtained from Vector Laboratories. AUY922 and MG132 were from MedChem Express.