MeT-5A, a human immortalized mesothelial cell line, was obtained from the American Type Culture Collection (ATCC; Manassas, VA) and maintained as described [44 (link)]. Six Japanese mesothelioma cell lines, ACC-MESO-1, ACC-MESO-4, Y-MESO-8A, Y-MESO-8D, Y-MESO-9 and Y-MESO-12, were established by Yoshitaka Sekido (Aichi Cancer Center Research Institute, Nagoya, Japan) [54 (link), 55 (link)]. NCI-H290 and NCI-H2452 were kindly provided by Dr. Adi F. Gazdar (Hamon Center for Therapeutic Oncology, University of Texas Southwestern Medical Center, Dallas, TX). NCI-H2052, NCI-H2373 and MSTO-211H were purchased from ATCC. Normal human lung fibroblast (NHLF) was purchased from Lonza (Basel, Switzerland). The mesothelioma cell lines and NHLF were grown in RPMI 1640 medium, supplemented with 10% fetal bovine serum (FBS) and 1% antibiotic-antimycotic solution (Invitrogen, Carlsbad, CA). All the cell lines were cultured in a 5% CO2 humidified atmosphere at 37 °C and used at 10-20 passages.
Nci h2373
Manufactured by American Type Culture Collection
Sourced in United States
The NCI-H2373 is a cell line derived from a human lung carcinoma. It is maintained in culture and available for research use.
Automatically generated - may contain errors
Lab products found in correlation
Nci h2052, by American Type Culture Collection (6 mentions)
Msto 211h, by American Type Culture Collection (5 mentions)
Met 5a, by American Type Culture Collection (4 mentions)
Nci h28, by American Type Culture Collection (4 mentions)
Nci h2452, by American Type Culture Collection (3 mentions)
Nci h226, by American Type Culture Collection (2 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Normal human lung fibroblast, by Lonza (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Antibiotic antimycotic solution, by Thermo Fisher Scientific (1 mentions)
Temozolomide, by Merck Group (1 mentions)
Olaparib, by Targetmol (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 culture medium, by Merck Group (1 mentions)
Met5a, by Merck Group (1 mentions)
7 protocols using nci h2373
1
Establishing Mesothelioma Cell Lines
2
Establishment of Japanese Mesothelioma Cell Lines
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Nineteen Japanese MM cell lines, namely ACC-MESO-1, -4, Y-MESO-8D, -9, -12, -14, -21, -22, -25, -26B, -27, -28, -29, -30, -45, -48, -61, -72, and -76, were established in our laboratory as reported previously and described elsewhere, and the cells at 10–15 passages were used for assays.28 (link),29 (link) Four MM cell lines including NCI-H28, NCI-H2052, NCI-H2373, and MSTO-211H, and one immortalized mesothelial cell line, MeT-5A, were purchased from ATCC (Rockville, MD, USA), and cells at 3–5 passages were used. NCI-H290 and NCI-H2452 were the kind gifts of Dr. Adi F. Gazdar (Hamon Center for Therapeutic Oncology, University of Texas Southwestern Medical Center, Dallas, TX, USA). All MM cell lines and MeT-5A were cultured as described in Data S1 . Malignant mesothelioma tissue samples from patients for the establishment of cell culture were obtained according to the Institutional Review Board’s approved protocol and with written informed consent from each patient.
3
Comprehensive Mesothelioma Cell Line Panel
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Fourteen PM cell lines were used in this study, of which MSTO-211H (211H, RRID: CVCL_1430), NCI-H28 (H28, RRID: CVCL_1555), NCI-H226 (H226, RRID: CVCL_1544), NCI-H2052 (H2052, RRID: CVCL_1518), NCI-H2373 (H2373, RRID: CVCL_A533), and NCI-H2452 (H2452, RRID: CVCL_1553) were purchased from the ATCC. ACC-MESO-1 (ACC1, RRID: CVCL_5113), ACC-MESO-4 (ACC4, RRID: CVCL_5114), Y-MESO-8A (Y8A, RRID: CVCL_5188), Y-MESO-9 (Y9, RRID: CVCL_5190), Y-MESO-12 (Y12, RRID: CVCL_5178), Y-MESO-14 (Y14, RRID: CVCL_5179), Y-MESO-25 (Y25, RRID: CVCL_5182), and Y-MESO-30 (Y30, RRID: CVCL_5187) were established in the laboratory of Dr. Yoshitaka Sekido and have been deposited in RIKEN BioResource Research Center (26 (link)). Met-5A (RRID: CVCL_3749), an epithelial cell line from the mesothelium, was purchased from ATCC. All cell lines were maintained in a humidified incubator at 37°C with 5% CO2 and were cultured in RPMI1640 medium with 10% FBS and 1% penicillin–streptomycin. Cell lines were authenticated using DNA fingerprinting and the cell lines were routinely checked for mycoplasma infection using the MycoAlertTM Mycoplasma detection Kit. AZD6738, an ATR inhibitor (27 (link)), ONO7475, a specific AXL and MER tyrosine kinase inhibitor (28 (link), 29 (link)), and NPS1034, a dual inhibitor of AXL and MET (30 (link)), were purchased from SelleckChem and ChemieTek.
4
Cell Line Maintenance and Experimental Treatments
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UM cell lines (92.1, Mel202, Mel270, OMM2.2, OMM2.3, OMM2.5, OMM1, OCM1, Mel285, and Mel290), provided by Dr. Martine Jager (Leiden University), were maintained as previously described [5 (link)]. Y‐MESO‐14 was a gift from Dr. Yoshitaka Sekido (Aichi Medical University), and NCI‐H2373 was purchased from ATCC. These two mesothelioma cell lines were maintained as previously described [27 (link)]. Human embryonic kidney 293T cells were from ATCC and cultured in Dulbecco’s modified Eagle’s medium with 10% fetal bovine serum. All media supplemented with 50 μg·mL−1 penicillin/streptomycin. Cells were maintained at 37 °C with 5% CO2 and confirmed to be free of mycoplasma. Olaparib (PARP inhibitor, TargetMol) and temozolomide (alkylating agent, Sigma‐Aldrich, Shanghai, China) were pre‐prepared as stock solution in DMSO and used at concentrations indicated in figures.
5
Establishment and Characterization of MM Cell Lines
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Nine Japanese MM cell lines including ACC‐MESO‐1, ‐4, Y‐MESO‐22, ‐27, ‐28, ‐30, ‐37, ‐45, and ‐72, were established in our laboratory, and cells at 10‐15 passages were used for each assay.15 (link) Five MM cell lines, including NCI‐H28, NCI‐H2052, NCI‐H2373, NCI‐H2452 and MSTO‐211H, and MeT‐5A (an immortalized mesothelial cell line), were purchased from the American Type Culture Collection. Cell line authentication of NCI‐H2052, NCI‐H2373, NCI‐H2452, and MeT‐5A cells was performed using short tandem repeat analysis. All MM cell lines and MeT‐5A cells were maintained in RPMI‐1640 culture medium (Sigma‐Aldrich) containing 5% fetal calf serum (FCS) in an atmosphere of 5% CO2 and 95% air at 37°C. 293FT cells were cultured in DMEM (Thermo Fisher Scientific) supplemented with 5% FCS.
6
HOMC-D4 Cell Line Establishment and Pleural Fluid Analysis
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HOMC‐D4 cell lines established in our laboratory35 were used for assays at passages 10–20. They were deposited and are now available from RIKEN BioResource Center. NCI‐H2052 and NCI‐H2373 cells were purchased from the American Type Culture Collection. All cell lines were cultured in RPMI‐1640 medium supplemented with 10% (v/v) fetal bovine serum (FBS) and 1% (v/v) antimicrobials at 37°C in a humidified incubator with 5% CO2. Pleural fluid samples from patients were collected and centrifuged at 3000 rpm for 10 min, and the supernatant was stored at −80°C until use. The research protocol was approved by the Ethics Committee of Aichi Cancer Center Research Institute.
7
Diverse Human MPM Cell Lines
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We examined 12 human MPM cell lines in this study. ACC-MESO-1, ACC-MESO-4, Y-MESO-9, Y-MESO-12, and Y-MESO-14 were established at the Aichi Cancer Research Center Institute [30 (link),31 (link)]. MSTO-211H, NCI-H28, NCI-H226, NCI-H2052, NCI-H2373, and NCI-H2452 were purchased from American Type Culture Collection (ATCC). The 3T3 (mouse fibroblast) cells were also obtained from ATCC. The cdk4/hTERT-immortalized normal human bronchial epithelial cell line HBEC3 was obtained from the Hamon Center (University of Texas Southwestern Medical Center, Dallas, TX, USA).
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