Hrp affinipure goat anti mouse igg

Dulbecco’s modified Eagle’s medium (DMEM) and fetal bovine serum (FBS) were purchased from Gibco (USA). BRB, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), dimethyl sulfoxide (DMSO), HAuCl₄. 3H₂O (≥99.9% trace metals basis), BSA, Triton X-100 and lipopolysaccharide (LPS) were purchased from Sigma-Aldrich (USA). RIPA lysis buffer, phenylmethanesulfonyl fluoride, hematoxylin-eosin (HE) staining kit and Nissl staining solution (methylene blue) were obtained from Solarbio (China). The antibodies to anti-CD86, anti-CD206, anti-Cleaved Caspase-3 and anti-TNF-α were purchased from Cell Signaling Technology (USA). Anti-Bax, anti-Bcl-2, anti-IL-1β and anti-IL-6 were purchased from Affinity (USA). Anti-β-Tubulin, anti-GAPDH, HRP Affinipure Goat Anti-Mouse IgG and HRP Affinipure Goat Anti-Rabbit IgG were purchased from Proteintech (USA). The Alexa Fluor^®568 goat anti-mouse/rabbit IgG and Alexa Fluor^®488 goat anti-mouse/rabbit IgG were purchased from Invitrogen (USA). 4,6-dimethyl-2-phenylindole (DAPI) was purchased from Abcam (UK). RAW 264.7 cells and VSC 4.1 cells were obtained from the American type culture collection.

Western blot methods were performed according to previously studied [21 (link)]. The accompanying antibodies were utilized for recognition in this experiment: antiphosphorylated AMPK (p-AMPK, 1 : 1000, Cell Signaling Technology, USA), anti-AMPK (1 : 1000, Cell Signaling Technology), anti-p-ACC (1 : 1000, Cell Signaling Technology), anti-ACC (1 : 1000, Cell Signaling Technology), anti-NLRP3 (1 : 1000, Cell Signaling Technology), anti-ASC (1 : 1000, Abcam, UK), anti-Caspase 1 (1 : 1000, Abcam), anti-IL-1β (1 : 1000, Abcam), anti-GAPDH (1 : 1000, Proteintech, USA), HRP Affinipure Goat Anti-Mouse IgG (1 : 1000, Proteintech), and HRP Affinipure Goat Anti-Rabbit IgG (1 : 1000, Proteintech). The outcomes were investigated with an UVP Electrophoresis Gel Imaging System (US) and ImageJ programming.