The lens opacity was first documented by a slit lamp (SL-D4; Topcon, Livermore, CA, USA) with 40× magnification every day for the first 12 days after UVB irradiation and then every 2 weeks. The mydriatic eye drop (0.5% tropicamide ophthalmic solution; NDC 1748-101-12; Akorn, Lake Forest, IL, USA) was given 5 minutes before the slit-lamp recording. Mice were anesthetized by isoflurane (46066-755-04; Aspen Veterinary Resources, Liberty, MO, USA) during the procedure. At the end of each experimental time point, the mice lenses were dissected and a darkfield image was taken with a Leica M80 microscope (Leica, Buffalo Grove, IL). Lastly, the lens capsule was dissected, and the capsular opacity was documented by a Leica M80 microscope and a Leica DMi1 phase-contrast microscope (Leica Microsystems, Wetzlar, Germany).
Dmi1 phase contrast microscope
Manufactured by Leica
Sourced in Germany
The Leica DMi1 is a phase-contrast microscope designed for laboratory applications. It provides high-contrast imaging of transparent samples without the need for staining or labeling. The microscope utilizes phase-contrast optics to enhance the visibility of details in unstained specimens.
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Lab products found in correlation
2 protocols using dmi1 phase contrast microscope
1
Quantifying UVB-induced Lens Opacity
2
Wound Scratch Assay for Cell Migration
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For the wound scratch assay, cells were grown in 6-well plates until they reached confluence. After 24 h incubation in serum-reduced medium, cell-free zones were created by scratching the cell layer with a P200 pipette tip. Images of the cell-free zone were captured immediately (0 h), 4 and 8 h after wounding, using a Leica DMi1 phase-contrast microscope (Leica Microsystems, Wetzlar, Germany). Between imaging periods, the cells were incubated at 37°C and 5% CO2. The area of the cell-free zone was measured using Digimizer image analysis software (MedCalc Software bvba, Ostend, Belgium). The closure of the cell-free area was calculated as follows: (area of cell-free zone at t0h - area of cell-free zone at txh)/area of cell-free zone at t0h.
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