Bel 7404
Manufactured by Genechem
Sourced in China
The BEL-7404 is a laboratory equipment used for cell culture applications. It is designed to provide a controlled environment for the growth and maintenance of cells. The device includes features for temperature, humidity, and gas regulation to support optimal cell culture conditions.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (7 mentions)
Smmc 7721, by Genechem (6 mentions)
Bel 7404, by Thermo Fisher Scientific (6 mentions)
Smmc 7721, by Thermo Fisher Scientific (5 mentions)
Bel 7402, by Genechem (4 mentions)
Hepg2, by Genechem (4 mentions)
Hep3b, by Genechem (4 mentions)
Rpmi 1640, by Thermo Fisher Scientific (4 mentions)
Bel 7402, by Thermo Fisher Scientific (4 mentions)
Sk hep 1, by Genechem (3 mentions)
Hep3b, by American Type Culture Collection (3 mentions)
Hep3b, by Thermo Fisher Scientific (3 mentions)
Scrambled sirna of urhc sirna con and urhc sirnas, by GenePharma (3 mentions)
Gv230 lentiviruses, by Genechem (3 mentions)
Lipofectamine 2000 reagent, by Thermo Fisher Scientific (3 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (3 mentions)
Hepg2, by Thermo Fisher Scientific (2 mentions)
Dulbecco s modi ed eagle medium dmem, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Bovogen (1 mentions)
Dulbecco modified eagle medium (dmem), by Corning (1 mentions)
10 protocols using bel 7404
1
Cultivation of Human Liver Cancer Cell Lines
2
Hepatocellular Carcinoma Cell Lines
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The HCC cell lines SMMC-7721 and BEL-7404 were obtained from Genechem (Shanghai, China). BEL-7404 and SMMC-7721 cells were maintained in RIPA-1640 and DMEM supplemented with 10% FBS, respectively. The cells were incubated at 37°C in humidified atmosphere containing 5% CO2. Additionally, 30 HCC tissue samples and matched ANLTs were obtained from Xijing Hospital (Shaanxi, China), and all participants provided written informed consent.
3
Hepatocellular Carcinoma Cell Lines
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The HCCLM3 and MHCC97H cell lines were gifts from the Liver Cancer Institute, Zhong Shan Hospital (Shanghai, China). One normal hepatocyte cell line (L02) and six HCC cell lines (Huh7, BEL7404, SMMC7721, SK-HEP-1, Hep3B, and BEL7402) were purchased from GeneChem (Shanghai, China) and cultured in medium recommended by GeneChem.
4
Establishing DEPDC1B Overexpression Cell Lines
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The human normal ovarian cell line IOSE-80 was obtained from Shanghai Jingyuan Co., Ltd. (Shanghai, China). The EOC cell lines OVCAR3 and SKOV3, and the human hepatocellular carcinoma cell line BEL-7404 were obtained from Shanghai GeneChem Co., Ltd. (Shanghai, China). The human lung carcinoma cell line A549 was purchased from Cobioer Biosciences Company (Nanjing, China). IOSE-80 and BEL-7404 cells were routinely maintained in RPMI 1640 medium (GIBCO, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS, GIBCO). OVCAR3 cells were maintained in RPMI 1640 medium supplemented with 20% FBS. SKOV3 cells were maintained in McCoy's 5a medium (GIBCO) supplemented with 10% FBS. A549 cells were incubated in F12K medium supplemented with 10% FBS. Lentiviral DEPDC1B overexpression vector was purchased from the GeneChem. To establish cell lines stably expressing a high level of DEPDC1B, lentivirus-infected cells were selected with 2 μg/mL of puromycin (GeneChem).
5
HCC Cell Lines Cultivation and KIF21B Silencing
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HCC cell lines Hep-G2, BEL7402, BEL-7404, and SMMC-7721 and the normal liver cell line Chang liver were purchased from Shanghai Genechem Co., Ltd. (Shanghai, China). BEL7402, BEL-7404, and Chang liver cells were cultured in Roswell Park Memorial Institute 1640 medium (Gibco, Thermo Fisher Scientific, Inc.) supplemented with 10% fetal bovine serum (Gibco, Thermo Fisher Scientific, Inc.). SMMC-7721 and HepG2 cells were maintained in Dulbecco’s modified Eagle’s medium (Invitrogen, Carlsbad, CA, United States) supplemented with 10% fetal bovine serum. The cell lines were cultured in a 37 °C incubator with 5% CO2. To further probe the role of KIF21B in HCC cells, KIF21B expression in BEL-7404 cells was silenced using lentivirus-mediated siRNA. In brief, cells were transfected for 24 h with lentiviral constructs expressing short hairpin RNA (shRNA) specific for KIF21B (shKIF21B; Shanghai Genechem Co., Ltd.) or control shRNA (shCtrl; Shanghai Genechem Co., Ltd.). Green fluorescence was used to estimate the efficiency of transfection. Stable knockdown cells were selected using puromycin.
6
Liver Cancer Cell Lines Cultivation
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The human liver cancer cell lines HepG2, SMMC7721, BEL-7404, SK-hep-1 and Huh7 were purchased from Shanghai Genechem Co., Ltd. (Shanghai, China). HCC-LM3, which is a highly invasive hepatocellular carcinoma cell line, was provided as a gift from the laboratory of the Eastern Hepatobiliary Surgery Hospital affiliated to the Second Military Medical University. HCC-LM3 cell line was approved by the Institute Ethics Committee at the Affiliated Hospital of Nantong University. The HCC-LM3, HepG2 and Huh7 liver cancer cell lines were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS; Gibco) at 37°C under 5% CO2. The liver cancer cell lines SMMC7721 and BEL-7404 were cultured in RPMI-1640 (Invitrogen, Carlsbad, CA, USA) medium with 10% FBS (Gibco) at 37°C under 5% CO2. The SK-hep-1 liver cancer cell line was cultured in MEM (Invitrogen, Carlsbad, CA, USA) supplemented with 10% FBS (Gibco) at 37°C under 5% CO2.
7
Culturing Human Liver Cell Lines
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The human normal hepatocyte cell lines L‐02 and the HCC cell lines Hep3B and Huh‐7 were purchased from the Shanghai Institute of Cell Biology at the Chinese Academy of Sciences (Shanghai, China). The HCC cell lines SMMC‐7721, Bel‐7402, Bel‐7404, SK‐Hep‐1, and HepG2 were obtained from Genechem Co., Ltd. (Shanghai, China). The HCC cell line MHCC‐97L was obtained as a gift from the First Affiliated Hospital of Xi'an Jiaotong University. MHCC‐97L, Huh‐7, and HepG2 cells were cultured in DMEM containing 10% FBS. L‐02, SMMC‐7721, Bel‐7402, and Bel‐7404 cells were cultured in RPMI‐1640 medium, while Hep3B and SK‐Hep‐1 cells were cultured in MEM medium containing 10% FBS. All media were supplemented with penicillin (100 U/ml) and streptomycin (100 U/ml). All cells were maintained in an incubator with a humidified atmosphere of 5% CO2 at 37°C.
8
Characterization of Hepatocellular Carcinoma Cell Lines
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The human HCC cell line Hep3B and 293T cells were purchased from ATCC. The normal human liver cell line MIHA was purchased from YaJi Biological (Shanghai, China). HCC cells Bel-7402 and Bel-7404 were obtained from Beina Biological (Beijing, China) in 2017. The SMMC-7721 was bought from Fenghui Biotechnologies Inc. (Hunan, China). MIHA, SMMC-7721, Bel-7402, and Bel-7404 cells were cultured in RPMI 1640 (Invitrogen, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS, Invitrogen). Hep3B and 293T were cultured in Dulbecco's modified Eagle medium (DMEM, Invitrogen) containing 10% FBS. All cells were incubated in a humidified atmosphere of 5% CO2 at 37°C. Dimethyl sulfoxide was purchased from Sigma-Aldrich (St. Louis, MO).
Scrambled siRNA of URHC (siRNA-Con) and URHC siRNAs were purchased from GenePharma (Shanghai, China). miR-5007-3p inhibitor, inhibitor negative control (NC inhibitor), miR-5007-3p mimic, and NC mimic were also purchased from GenePharma (Shanghai, China). Full-length URHC cDNA was subcloned into GV230 lentiviruses (Genechem, Shanghai, China) and infected into Bel-7404 and Hep3B cells to generate URHC-overexpressing cells. Lipofectamine 2000 Reagents (Invitrogen Co., USA) were used for cell transfections.
Scrambled siRNA of URHC (siRNA-Con) and URHC siRNAs were purchased from GenePharma (Shanghai, China). miR-5007-3p inhibitor, inhibitor negative control (NC inhibitor), miR-5007-3p mimic, and NC mimic were also purchased from GenePharma (Shanghai, China). Full-length URHC cDNA was subcloned into GV230 lentiviruses (Genechem, Shanghai, China) and infected into Bel-7404 and Hep3B cells to generate URHC-overexpressing cells. Lipofectamine 2000 Reagents (Invitrogen Co., USA) were used for cell transfections.
9
Cell Culture and Transfection Protocols
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The human HCC cell line Hep3B and 293T cells were purchased from ATCC. The normal human liver cell line MIHA was purchased from YaJi Biological (Shanghai, China). HCC cells Bel-7402 and Bel-7404 were obtained from Beina Biological (Beijing, China) in 2017. The SMMC-7721 was bought from Fenghui Biotechnologies Inc (Hunan, China). MIHA, SMMC-7721, Bel-7402, and Bel-7404 cells were cultured in RPMI 1640 (Invitrogen, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS, Invitrogen). Hep3B and 293T were cultured in Dulbecco's modi ed Eagle medium (DMEM, Invitrogen) containing 10% FBS.
All cells were incubated in a humidi ed atmosphere of 5% CO 2 at 37°C. Dimethyl sulfoxide was purchased from Sigma-Aldrich (St. Louis, MO). Scrambled siRNA of URHC (siRNA-Con) and URHC siRNAs were purchased from GenePharma (Shanghai, China). miR-5007-3p inhibitor, inhibitor negative control (NC inhibitor), miR-5007-3p mimic and NC mimic were also purchased from GenePharma (Shanghai, China). Full-length URHC cDNA was subcloned into GV230 lentiviruses (Genechem, Shanghai, China) and infected into Bel-7404 and Hep3B cells to generate URHC-overexpressing cells. Lipofectamine 2000 Reagents (Invitrogen Co, USA) were used for cell transfections.
All cells were incubated in a humidi ed atmosphere of 5% CO 2 at 37°C. Dimethyl sulfoxide was purchased from Sigma-Aldrich (St. Louis, MO). Scrambled siRNA of URHC (siRNA-Con) and URHC siRNAs were purchased from GenePharma (Shanghai, China). miR-5007-3p inhibitor, inhibitor negative control (NC inhibitor), miR-5007-3p mimic and NC mimic were also purchased from GenePharma (Shanghai, China). Full-length URHC cDNA was subcloned into GV230 lentiviruses (Genechem, Shanghai, China) and infected into Bel-7404 and Hep3B cells to generate URHC-overexpressing cells. Lipofectamine 2000 Reagents (Invitrogen Co, USA) were used for cell transfections.
10
Cell Culture and Transfection Protocols
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The human HCC cell line Hep3B and 293T cells were purchased from ATCC. The normal human liver cell line MIHA was purchased from YaJi Biological (Shanghai, China). HCC cells Bel-7402 and Bel-7404 were obtained from Beina Biological (Beijing, China) in 2017. The SMMC-7721 was bought from Fenghui Biotechnologies Inc (Hunan, China). MIHA, SMMC-7721, Bel-7402, and Bel-7404 cells were cultured in RPMI 1640 (Invitrogen, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS, Invitrogen). Hep3B and 293T were cultured in Dulbecco's modi ed Eagle medium (DMEM, Invitrogen) containing 10% FBS.
All cells were incubated in a humidi ed atmosphere of 5% CO 2 at 37°C. Dimethyl sulfoxide was purchased from Sigma-Aldrich (St. Louis, MO). Scrambled siRNA of URHC (siRNA-Con) and URHC siRNAs were purchased from GenePharma (Shanghai, China). miR-5007-3p inhibitor, inhibitor negative control (NC inhibitor), miR-5007-3p mimic and NC mimic were also purchased from GenePharma (Shanghai, China). Full-length URHC cDNA was subcloned into GV230 lentiviruses (Genechem, Shanghai, China) and infected into Bel-7404 and Hep3B cells to generate URHC-overexpressing cells. Lipofectamine 2000 Reagents (Invitrogen Co, USA) were used for cell transfections.
All cells were incubated in a humidi ed atmosphere of 5% CO 2 at 37°C. Dimethyl sulfoxide was purchased from Sigma-Aldrich (St. Louis, MO). Scrambled siRNA of URHC (siRNA-Con) and URHC siRNAs were purchased from GenePharma (Shanghai, China). miR-5007-3p inhibitor, inhibitor negative control (NC inhibitor), miR-5007-3p mimic and NC mimic were also purchased from GenePharma (Shanghai, China). Full-length URHC cDNA was subcloned into GV230 lentiviruses (Genechem, Shanghai, China) and infected into Bel-7404 and Hep3B cells to generate URHC-overexpressing cells. Lipofectamine 2000 Reagents (Invitrogen Co, USA) were used for cell transfections.
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