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2 protocols using creatine

1

Quantification of Bioactive Compounds

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Chicken breast (recessive white chicken) and turmeric were purchased from the local supermarket (Wuhan, Hubei, China). Norharman and harman were obtained from Toronto Research Chemicals Inc. (Downsview, Ont., Canada). Curcumin, tryptophan, phenylalanine, and creatine were purchased from Shanghai yuanye Bio‐Technology Co., Ltd (Shanghai, China). Methanol and acetonitrile (High Performance Liquid Chromatography (HPLC) grade) were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Acetic acid, ammonium acetate, sodium hydroxide, ammonium hydroxide, dichloromethane, diethylene glycol, and hydrochloric acid glucose were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). The MCX SPE column (Oasis® MCX 3cc, 60 mg, Extraction Cartridges) was obtained from Waters Corporation (Waters, Milford, MA, USA).
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2

Quantitative Analysis of PhIP and Metabolites

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PhIP standard was acquired from Toronto Research Chemicals (North York, Ontario, Canada). Glucose, creatinine, creatine, phenylalanine, and phenylacetaldehyde were obtained from Yuanye Biotechnology (Shanghai, China), of which the purity is over 98%. Methanol, acetonitrile, and formic acid were provided by Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China), with a purity of 99.8%.
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