Acetonitrile lc ms reagent

Dulbecco’s Modified Eagle Medium (DMEM) containing phenol red, penicillin–streptomycin, EDTA-trypsin, and Dulbecco’s phosphate buffer solution (DPBS) was purchased from Gibco BRL Life Technologies (Invitrogen™, Paisley, UK). Fetal bovine serum (FBS) was purchased from HyClone, USA. 3-(4,5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt (MTS) was purchased from Promega, USA. Hydrogen peroxide, 2′,7′dihydrofluorescein diacetate (DCFH-DA), chlorpromazine hydrochloride (CPZ), QA, neochlorogenic acid (CAS No. 906-33-2) with IUPAC name 3-O-caffeoylquinic acid (3-CQA), and cryptochlorogenic acid (CAS No. 905-99-2) with IUPAC name 4-O-caffeoylquinic acid (4-CQA) were purchased from Sigma-Aldrich, United Kingdom. Acetonitrile (LC–MS reagent) was purchased from RCI Labscan (Bangkok, Thailand). Water type I was purified from MilliQ^® Integral 3 system (Millipore, Bedford, MA, USA). Formic acid (analytical grade) was purchased from Merck (Darmstadt, Germany). A 0.45 mm nylon disposable membrane filter and syringe filter were supplied from Vertical Chromatography, Thailand. Other unmarked reagents were of analytical grade.