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5 protocols using glucose c6h12o6

1

Electrochemical Characterization of Aluminum

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Aluminum foil (Al, product number: 326860-3.6G), glucose (C6H12O6, product number: PHR1000-1G), magnesium chloride (MgCl2, product number 449172-10G), oxalic acid (C2H2O4, product number: 247537-500G), perchloric acid (HClO4, product number: 311421-250ML), phosphoric acid (H3PO4, product number: 695017-500ML), sodium hydroxide (NaOH, product number: 306576-100G), and deionized water (H2O, product number: W4502-1L with a resistivity of 18 MΩ) were procured from Sigma-Aldrich (St. Louis, MI, USA). These reagents were employed as provided, without any further purification, ensuring the consistency of experimental conditions.
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2

Synthesis and Characterization of Silver Nanoparticles

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AgNO3 and glucose (C6H12O6) were supplied by Sigma Aldrich. Pure and deionized water was employed to wash and purify the NPs samples. Glass beakers, a magnetic stirrer and a glass stirrer were utilized to prepare the AgNPs samples. In every examination, 100 mL of the obtained solution was utilized.
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3

Colorimetric Glucose Assay with R6G

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All chemicals used were reagent grade or higher. All solutions were prepared with reverse osmosis (RO) water. Sulfuric acid (H2SO4) was purchased from Honeywell Fluka™. Sodium hydroxide (NaOH) was purchased from SHIMAKYU Chemical. Glucose (C6H12O6) and R6G were purchased from Sigma-Aldrich.
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4

Electrochemical Detection of Glucose in Blood

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All the reagents and chemicals used were of analytical grade. Ammonium molybdate tetrahydrate (NH4)6 Mo7O24.4H2O, selenium metal powder (99.9%), hydrazine hydrate (N2H4, 80%), nickel chloride hexahydrate (NiCl2.6H2O), sodium hydroxide (NaOH), sodium oxalate (Na2C2O4) and glucose (C6H12O6)) were purchased from Sigma-Aldrich, Sisco Research Laboratories, Thermo Fisher Scientific and Loba Chemie. These chemicals were used without any further purifications. All solutions were prepared with Mili-Q distilled water (18.2 MΩ cm @ 25 ± 2 °C).
Electrochemical measurements were carried out by using the electrochemical workstation (Model: CHI-760E) from CH Instruments, Austin, TX, USA. Electrochemical studies were performed in a standard electrochemical cell using a three-electrode system with NiO/MoSe2/GCE as the working electrode, Ag/AgCl (3M KCl) as the reference electrode and platinum wire as the counter (auxiliary) electrode. The blood serum samples were received from the SRM Medical College Hospital and Research Centre, Kattankulathur, Tamil Nadu. All the experiments were carried out in accordance with the relevant guidelines and regulations. The SRMIST ethics committee was approved the experiments (Ref. No: 002/HYC/IEC/2018). Informed consents were obtained from the human participants of this study.
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5

Electrochemical Detection of 5-Fluorouracil

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All the chemicals and reagents [thiophene monomer (C4H4S) (≥99% SRL), ferric chloride (FeCl3) (≥98% AR), boron nitride bulk (h-BN) (≥99% SRL), sodium hydroxide (NaOH) (≥98% FISHER), acetonitrile, ethanol, methanol, 5-fluorouracil (C4H3FN2O2) (≥99% SRL), uric acid (C5H4N4O3) (≥99% SRL), lactose (C12H22O11) (≥98% SRL), oxalic acid (≥99.5% AR), ascorbic acid (C6H8O6) (≥99.7% SRL), dopamine (C8H11NO2) (≥99% SRL), l-cysteine (C3H7NO2S) (≥99% SRL), glucose (C6H12O6) (≥99% SRL), potassium ferricyanide (C6N6FeK3) (≥99% SRL), potassium ferrocyanide (C6FeK4N6) (≥99% SRL), potassium chloride (KCl) (99.8% Fisher), monosodium hydrogen phosphate (NaH2PO4) (≥99% SRL), and disodium hydrogen phosphate (Na2HPO4) (≥99% SRL)] are purchased in Sigma Aldrich and Alfa Aesar. To create a stock solution, ultrapure deionized water was used. NaH2PO4 and Na2HPO4 were combined to create 0.1 M phosphate buffer (PBS) (pH = 7.0) for the detection of 5-Fu. The human blood serum sample was collected from SRM Medical College Hospital and Research Center, Kattankulathur, Tamil Nadu, for real sample analysis. All the experiments were done by using Milli-Q-water (18.2 MΩ cm @ 25 ± 2 °C).
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