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Cd4 brilliant violet 650

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The CD4-Brilliant Violet 650 is a fluorochrome-conjugated antibody that binds to the CD4 surface antigen. It is designed for use in flow cytometry applications to identify and analyze CD4+ cells in biological samples.

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Lab products found in correlation

Cd3 cd28 coated beads, by Thermo Fisher Scientific (1 mentions) Monensin, by Thermo Fisher Scientific (1 mentions) Ki67 alexa fluor 647, by BioLegend (1 mentions) Cd8α pacific blue, by BioLegend (1 mentions) Cd49d pe, by BioLegend (1 mentions) Cd127 pe cy7, by BioLegend (1 mentions) Cd4 apc cy7, by BD (1 mentions) Cd4 pe cy7, by BD (1 mentions) Cd4 fitc, by BioLegend (1 mentions) Cd127 pe, by BioLegend (1 mentions) Cd24 fitc, by BioLegend (1 mentions) Cd4 alexa fluor 700, by BioLegend (1 mentions) Cd62l alexa fluor 700, by BioLegend (1 mentions)

Spelling variants of this product

Brilliant Violet 650 (10 citations) Brilliant violet 650-conjugated CD4 (3 citations) Anti-CD4 Brilliant Violet 650 (3 citations) CD4-Brilliant Violet 650 (clone OKT4; (3 citations)

2 protocols using cd4 brilliant violet 650

1

TIGIT Modulates T Cell Cytokine Production

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PBMCs were activated for 24 hours with CD3/CD28 coated beads (ThermoFisher, 111.31D) with or without recombinant human TIGIT Fc chimera protein, or the respective recombinant human IgG1 Fc control (100 µg/mL, R&D Systems, 7898-TG-050 and 110-HG). Monensin (ThermoFisher, 00-4505-51) was added during the final four hours of culture. Intracellular cytokines were stained with anti-human IFNγ-FITC (ThermoFisher,11-7319-82), IL-21-PE (ThermoFisher, 12-7219-42), IL-10-PerCP-eFluor710 (ThermoFisher, 46-7108-42) and IL-4-APC (ThermoFisher, 17-7049-42). For surface marker expression TIGIT-PE-Cyanine7 (ThermoFisher, 25-9500-42), CD3-AF700 (ThermoFisher, 56-0038-42) and CD4-Brilliant Violet 650 (Biolegend, 300536) were used.
Catakovic K., Gassner F.J., Ratswohl C., Zaborsky N., Rebhandl S., Schubert M., Steiner M., Gutjahr J.C., Pleyer L., Egle A., Hartmann T.N., Greil R, & Geisberger R. (2017). TIGIT expressing CD4+T cells represent a tumor-supportive T cell subset in chronic lymphocytic leukemia. Oncoimmunology, 7(1), e1371399.
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2

Multiparameter Flow Cytometry Antibody Panel

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The antibodies that were used for flow cytometry were purchased from BD Pharmingen: CD4 PE-Cy7 (RM4-5) Cat# 552775, CD4 APC-Cy7 (GK1.5) Cat# 552051, CD4 Alexa Fluor 700 (RM4-5) Cat# 557956, CD4 FITC (H129.19) Cat# 553651, CD8α Pacific Blue (53-6.7) Cat# 558106, CD49d PE (R1-2) Cat# 553157, Ki67 Alexa Fluor 647 (B56) Cat# 558615, KLRG1 APC (2F1/KLRG1) Cat# 553157, Ly6c FITC (MR5-2) Cat# 553186, TCR Vα2 PE (B20.1) Cat# 553289, TCR Vα8.3 FITC (B21.14) Cat# 553376, TCR Vβ8.1/8.2 FITC (MR5-2) Cat# 553185, TCRβ APC (H57-597) Cat# 553174, TCRβ PE (H57-597) Cat# 553171, Biolegend: CD4 Brilliant Violet 650 (RM4-5) Cat# 100545, CD5 Brilliant Violet 510 (53-7-3) Cat# 100627, CD8α Brilliant Violet 421 (53-6.7) Cat# 558106, CD8α Brilliant Violet 510 (53-6.7) Cat# 558106, CD24 FITC (M1/69) Cat# 101806, CD44 Brilliant Violet 650 (IM7) Cat# 103049, CD44 PerCP-Cy5.5 (IM7) Cat# 103032, CD49d PE-Cy7 (R1-2) Cat# 103618, CD62L Alexa Fluor 700 (MEL14) Cat# 104426, CD122 FITC (TM-β1) Cat# 123207, CD127 PE-Cy7 (A7R34) Cat# 135015, CD127 PE (A7R34) Cat# L34975, CD218a Alexa Fluor 647 (BG/IL18RA) Cat# 132903, CX3CR1 PE-Cy7 (SA011F11) Cat# 149016, IFNγ Alexa Fluor 700 (XMG1.2) Cat# 505824, TCR Vα8.3 PE (B21.14) Cat# 127708, TCR γ/δ PerCP-Cy5.5 (GL3) Cat# 118117, or eBioscience: CD122 PerCP-eFluor 710 (TM-β1) Cat# 46-1222-80, EOMES Alexa Fluor 488 (Dan11mag) Cat# 53-4875-80.
Moudra A., Niederlova V., Novotny J., Schmiedova L., Kubovciak J., Matejkova T., Drobek A., Pribikova M., Stopkova R., Cizkova D., Neuwirth A., Michalik J., Krizova K., Hudcovic T., Kolar M., Kozakova H., Kreisinger J., Stopka P, & Stepanek O. (2021). Phenotypic and clonal stability of antigen-inexperienced memory-like T cells across the genetic background, hygienic status, and aging. Journal of immunology (Baltimore, Md. : 1950), 206(9), 2109-2121.
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