Anti cd19 alexa 700

Manufactured by Thermo Fisher Scientific

The Anti-CD19-Alexa 700 is a fluorescently labeled antibody that binds to the CD19 cell surface antigen. It is designed for use in flow cytometry applications.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

Anti-CD19 (33 citations)

2 protocols using anti cd19 alexa 700

Cd244 Knockout Mice for Immunological Studies

Check if the same lab product or an alternative is used in the 5 most similar protocols

C57BL/6 (WT) mice were purchased from The Jackson Laboratories (Bar Harbor, ME).
Cd244^-/- mice on the C57BL/6 background [12 (link)], were kindly provided by Dr. Raymond Welsh (University of Massachusetts Medical Center, Worcester, MA). Mice were maintained in the Translational Biomedical Research Center of the Medical College of Wisconsin and both genders were used for experiments in an age (6–12 week) and gender-matched manner. anti-CD45R-PE-Texas Red, anti-CD45R-PE, anti-GL7-FITC and anti-CD5-APC were purchased from BD Biosciences (San Jose, CA). CD21-eFluor 450, anti-CD23-PE-Cy7, anti-CD93-biotin, anti-CD4-APC-eFluor 780, anti-CD8-PE-Cy7, anti-TCRβ-FITC, anti-TCRβ-PE, anti-CD11b-Alexa Fluor 488, anti-CD244, anti-Foxp3-PE, anti-CD19-Alexa 700, anti-CD4-PE, anti-IgG-FITC and Streptavidin PE-Cy5.5 were purchased from eBioscience (San Diego, CA). Anti-CD11b-Brilliant Violet 605, anti-CD11c-PE, anti-NK1.1-APC, anti-IgM-APC Cy7, anti-IgD-Pacific Blue, anti-CD38-Alexa Fluor 647, anti-CD138-APC and rat anti-mouse IgG_2a-biotin were purchased from Biolegend (San Diego, CA). Anti-IgM-FITC and the SBA Clonotyping System-B6/C57J-HRP were purchased from Southern Biotech (Birmingham, AL). 4-Hydroxy-3-nitrophenylacetyl (NP)-ficoll, NP-Chicken Gamma Globulin (CGG), NP(24)-PE and NP-BSA were purchased from Biosearch Technologies (Novato, CA).

Ray A., Yuan C.Y., Miller N.M., Mei H, & Dittel B.N. (2015). 2B4 Is Dispensable for T-Dependent B Cell Immune Responses, but Its Deficiency Leads to Enhanced T-Independent Responses Due to an Increase in Peritoneal Cavity B1b Cells. PLoS ONE, 10(8), e0137314.

+ Open protocol

+ Expand

Immunization and Analysis of Arthritis in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

Flt3L^−/−, WT and Batf3^−/− mice were immunized as previously described [23 (link)] and were inspected three times a week for signs of arthritis by two independent observers. All mice were sacrificed on day 43 after CIA induction. Blood, LNs and paws were harvested for analysis. Single-cell suspensions were obtained and after erythrocyte lysis (red blood cell lysis buffer, 2 min at RT; Sigma), cells were stained with the indicated fluorochrome-conjugated antibodies for surface markers and intracellular cytokines. LN cells were stained using the following markers: anti-TNF (APC, eBioscience), anti-IL-2 (APC, eBioscience), anti-IL-17 (Alexa 488, eBioscience), anti-IFNγ (PercP Cy5.5, eBioscience), anti-IL10 (PE, eBioscience), anti-GM-CSF (PE, eBioscience), anti-B220 (PerCP, eBioscience), anti-CD19 (Alexa 700, eBioscience), anti-GL7 (biotin, eBioscience), anti-IgD (PE, BD Pharmingen), anti-CD38 (FITC, eBioscience), anti-CD95 (APC, eBioscience) and streptavidin (PE-Cy7, eBioscience). Synovial cells were isolated and stained using antibodies against CD11c (PE-Cy7, eBioscience), MHCII (APC-Cy7, eBioscience), CD11b (Alexa 700) and CD103 (FITC, eBioscience). Serum levels of antibodies against chicken collagen type II (cCII) were measured by ELISA. Further details are described in supplementary methods.

Ramos M.I., Garcia S., Helder B., Aarrass S., Reedquist K.A., Jacobsen S.E., Tak P.P, & Lebre M.C. (2020). cDC1 are required for the initiation of collagen-induced arthritis. Journal of Translational Autoimmunity, 3, 100066.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!