The analysis was performed on a Model BX61 Fluorescence Microscope (Olympus Corp., Tokyo, Japan), Spinning Disk Olympus (DSU) system, coupled to an ORCA-R2 camera (Hamamatsu Photonics KK, Japan) and CellSens Dimension software v1.9 (Olympus Corp., Tokyo, Japan). Fluorescence emission was obtained by excitation with a xenon lamp. Emission was collected with a DAPI filter (450 to 500 nm).
Model bx61 fluorescence microscope
Manufactured by Olympus
Sourced in Japan
The Olympus BX61 is a fluorescence microscope designed for advanced microscopy applications. It features a high-intensity light source and specialized optics to enable the observation and analysis of fluorescently labeled samples.
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2 protocols using model bx61 fluorescence microscope
1
Fluorescence Microscopy of DAPI-Stained Samples
2
Immunofluorescence Staining of NPCs
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NPCs were plated on coverslips and fixed with 4% paraformaldehyde at 4°C for 15 min. Samples were permeabilized with 0.1% Triton X-100 dissolved in blocking buffer for 15 min and blocked with blocking buffer comprising 1% bovine serum albumin and 3% fetal bovine serum in PBS for 30 min at 20-25°C temperature. Coverslips were incubated overnight at 4°C with primary antibodies. After three washes, the coverslips were incubated with secondary antibodies conjugated with donkey anti-mouse or donkey anti-rabbit IgG for 1 h at 20-25°C temperature. The coverslips were mounted using Vectashield (Vector Laboratories, Burlingame, CA, USA), and cells were imaged using a model Bx61 fluorescence microscope (Olympus, Tokyo, Japan).
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