Sodium piruvate

Vero cells were grown in Dulbecco´s modified Eagle´s medium (DMEM) supplemented with 10% inactivated fetal bovine serum (FBS), 2 mM L-glutamine and 100 U mL -1 penicillin/streptomycin. Human neuroblastoma SH-SY5Y cells were obtained from American Type Culture Collection (ATCC) and were grown in Minimum Essential Medium (MEM) (Inlab Diagnóstica, Sao Paulo, Brazil) supplemented with Ham's F12 Nutrient Mixture (Sigma, St. Louis, USA), 1 mM sodium piruvate (Sigma, St. Louis, USA), 1% MEM non-essential amino acids (Gibco, Carlsbad, USA), 100 U/ml penicillin, 0.1 g/ml streptomycin, 2 mM L-glutamine and 10% (vol/vol) fetal bovine serum (FBS), at 37 °C with 5% CO 2 .
The Brazilian ZIKV strain, named ZIKV/H.sapiens/Brazil/PE243/2015 (abbreviated to ZIKV PE243; GenBank Acession number KX197192.1), was isolated from a patient who had the classical ZIKV exanthematous illnesses, without neurological signs [22] . The ZIKV PE243 strain was propagated and titrated on Vero cells. Viral titration was performed by the standard TCID 50 method [23] and expressed as log 10 TCID 50 mL -1 .
The thiopurine nucleoside analogue 6MMPr and the control ribavirin were purchased from Sigma-Aldrich (Saint Louis, USA). Stock solution of the compound was prepared in Milli-Q H 2 O, sterilized by filtering through a 0.22 µM Millipore filter, and stored at -20 °C.