Ultimate 3000rs uhplc instrument

Chromatographic separation was accomplished with a Dionex Ultimate 3000RS UHPLC instrument, equipped with a Thermo Accucore C₁₈ (100 mm × 2.1 mm i. d., 2.6 μm) analytical column for the separation of compounds. Water (A) and methanol (B) containing 0.1% formic acid were employed as mobile phases, respectively. The total run time was 70 min for the elution profile. Mass spectrum analysis was carried out using a Thermo Q-Exactive Orbitrap mass spectrometer (Thermo Scientific, Waltham, MA, USA) equipped with an electrospray ionisation probe interface in positive and negative-ion mode. All detailed analytical conditions have been published [62 (link)].

The total phenolic and flavonoid contents were determined using the Folin–Ciocalteu and aluminium chloride (AlCl₃) assays, respectively.^21,22 Results were expressed as gallic acid (mg GAEs per g extract) and rutin equivalents (mg REs per g extract) for respective assays.
Chromatographic separation was accomplished with a Dionex Ultimate 3000RS UHPLC instrument, equipped with Thermo Accucore C18 (100 mm × 2.1 mm i. d., 2.6 μm) analytical column for separation of compounds. Water (A) and methanol (B) containing 0.1% formic acid were employed as mobile phases, respectively. The total run time was 70 minutes, the elution profile and all exact analytical conditions have been published.^{23 (link)}

Spectrophotometric methods were used to determine total phenolic and flavonoid content as conducted in earlier papers. Standard equivalents (gallic acid equivalent: GAE for phenolic and rutin equivalent: RE for flavonoid) were used to explain the contents in the plant extracts [23 (link)].
The UHPLC/MS/MS technique was used to analyze the different extracts. Chromatographic separation was accomplished with a Dionex Ultimate 3000RS UHPLC instrument, equipped with a Thermo Accucore C18 (100 mm × 2.1 mm i. d., 2.6 μm) analytical column for the separation of compounds. Water (A) and methanol (B) containing 0.1% formic acid were both employed for mobile phases. The total run time was 70 min. A Thermo Q Exactive mass spectrometer was used to detect the separated components. All extracts were performed in two chromatographic runs with the recording of mass spectra in positive and negative ion mode, and protonated [M+H]⁺ or deprotonated molecules [M-H]^- and their fragments were recorded.The elution profile and all exact analytical conditions were published [24 (link)].