Nod scid il2rgammanull nsg female mice

As a mouse model we used 6-8 weeks old NOD-scid IL2Rgammanull (NSG) female mice obtained from Jackson Laboratories and bred in-house for experimental cohorts. All animal experiments were approved by the Animal Welfare Committee of The Netherlands Cancer Institute (NKI) in accordance with national guidelines. Animals were maintained in the animal department of the NKI, housed in individually ventilated cages (IVC) under specific pathogen-free (SPF) conditions, and received food and water ad libitum.

Xenograft animal experiments were approved by Institutional Animal Care and Use Committee of the University of Western Ontario (2015-65) and carried out in accordance with approved guidelines. NOD.CB17-Prkdc/NCrCrl (NOD/SCID) female mice from Charles River Laboratories (#394) (8–10 weeks old) were inoculated by intraperitoneal injection with 1 × 10⁶ cells in 150 µL of PBS.
NOD-scid IL2Rgammanull (NSG) female mice from Jackson Laboratory (#005557) and NOD.CB17-Prkdc/NCrCrl (NOD/SCID) female mice from Charles River Laboratories (Laval, Quebec, Canada; catalogue #394) (8–10 weeks old) were injected subcutaneously and bilaterally with 1 × 10⁷ cells in 150 µL of PBS mixed with ECM Gel from Engelbreth–Holm–Swarm murine sarcoma at a 1:1 ratio. Mice were sacrificed 85 and 140-days post-injection respectively and examined for tumor formation. Small white nodules were excised and digested in 30 µg/µL of collagenase from Clostridium histolyticum (C6885; MilliporeSigma, Oakville, ON, Canada) at 37 °C for one hour and the resultant cells placed in culture with Dulbecco’s modified Eagle medium/Ham’s F12 and supplemented with 10% FBS to recover resident cancer cells.