The levels of IL-1β, IL-6 and IL-8 were quantified in the plasma samples from study subjects by ELISA method using the Human IL-1β Instant ELISA Kit (Invitrogen, Waltham, Massachusetts, USA, Catalog Numbers: BMS224INST) and the Human IL-6 Instant ELISA Kit (Invitrogen, Waltham, Massachusetts, USA, Catalog Numbers: BMS213INST), and the Human IL-8 Instant ELISA Kit (Invitrogen, Waltham, Massachusetts, USA, Catalog Numbers: BMS204-3INST), respectively. The procedures were followed according to the manufacturer's instructions.
Human il 6 instant elisa kit
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Human IL-6 Instant ELISA Kit is a quantitative test used to measure the concentration of interleukin-6 (IL-6) in human samples. It provides a rapid and convenient method for the detection and quantification of IL-6 levels.
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4 protocols using human il 6 instant elisa kit
1
Quantifying Inflammatory Cytokines by ELISA
2
Quantifying Cytokine Levels in Smoke-Exposed Cells
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The concentration of IL6 and IL8 cytokines were measured in UltraCULTURE™ medium used for a 24 h cell recovery after the smoke exposure by the Human IL-6 Instant ELISA Kit and Human IL-8 Instant ELISA Kit (Invitrogen™). Each exposure condition was assayed in duplicate. The assays were carried out following the manufacturers’ instructions.
3
Serum TPO and IL-6 Quantification
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Serum samples were prepared by centrifugation at 1000 g for 15 min at room temperature and stored in a − 80 °C freezer until examination. To determine the levels of human TPO and IL-6, ELISAs were performed with the Human Thrombopoietin Quantikine ELISA Kit (R&D Systems) and the IL-6 Human Instant ELISA Kit (Invitrogen), respectively, according to the manufacturer’s instructions.
4
Cytokine Secretion Modulation by miR-4646-5p
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To determine the impact of miR-4646-5p on the secretion of the two cytokines granulocyte colony-stimulating factor (G-CSF) and interleukin-6 (IL-6), Enzyme-linked Immunosorbent Assays (ELISAs) were performed on the supernatant of SUM159 and MDA-MB-231 cells using the Human G-CSF Instant ELISA™ Kit (Invitrogen, Thermo Fisher Scientific) and IL-6 Human Instant ELISA™ Kit (Invitrogen, Thermo Fisher Scientific), respectively. Cells were transfected with 10 nM miR-4646-5p mimic or control, and after 24 h, growth medium was replaced by serum-free medium. After another 40 h, supernatant was harvested and cleared by centrifugation. A total of 50 μL supernatant was used per well in technical duplicates. Standards and blank were prepared according to the manufacturer’s instructions. After 3 h of incubation, wells were washed 6 times, and substrate solution was added according to instructions. Absorbances were read at 450 nM and 620 nM as reference using a SPECTROstar Omega spectrophotometer (BMG Labtech).
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