Cell proliferation was assessed using Click-iTTM Plus EdU flow Cytometry Assay Kit (Thermo Scientific), according to the manufacturer’s instructions. Briefly, 10 µM 5-ethynyl-2’-deoxyuridine (EDU) was added to adherent subconfluent HUVEC cultures and cells were incubated in HUVEC medium for 24 h at 37°C. Cell cycle analysis was determined by flow cytometry with 488 nm excitation (Beckton Dickinson).
488 nm excitation
Manufactured by BD
The 488 nm excitation is a type of lab equipment that provides a specific wavelength of light for use in various scientific applications. This equipment is designed to produce a laser or other light source with a wavelength of 488 nanometers, which is commonly used in fluorescence microscopy, flow cytometry, and other analytical techniques.
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2 protocols using 488 nm excitation
1
EdU-based HUVEC Proliferation Assay
2
EdU-based Flow Cytometry for Cell Proliferation
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Cell proliferation was assessed using a Click-iTTM Plus EdU flow Cytometry Assay Kit (Thermo Scientific), according to the manufacturer’s instructions. Briefly, 10 µM 5-ethynyl-2′-deoxyuridine (EdU) was added to adherent subconfluent HUVEC cultures at 72 h after siRNA transfection and cells were incubated in HUVEC medium for 24 h at 37 °C. Cell cycle analysis was determined by flow cytometry with 488 nm excitation (Beckton Dickinson).
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