Sealsafe plus mouse

Adult male and female C57BL/6NCrSlc mice were purchased from Japan SLC (Hamamatsu, Japan) for breeding stock. All mice were maintained in individual 40.5 × 20.5 × 18.5-cm ventilated cages (Sealsafe Plus Mouse; Tecniplast,
Buguggiate, Italy) under controlled temperature (23 ± 2°C) and humidity (50 ± 10%) on a 12-hr light/dark cycle at the Kobe University Life-Science Laboratory with ad libitum access to water and a pellet diet
(DC-8; Clea Japan, Tokyo, Japan). This study was approved by the Institutional Animal Care and Use Committee (Permission #24-10-03) and carried out according to the Kobe University Animal Experimental Regulations.

Male C57BL/6NCrSlc mice (3 weeks old) were purchased from Japan SLC (Hamamatsu, Japan). All mice were maintained in 40.5 × 20.5 × 18.5 cm individually ventilated cages (Sealsafe Plus Mouse;
Tecniplast, Buguggiate, Italy) under controlled temperature (23 ± 2°C) and humidity (50 ± 10%) conditions and on a 12-hr light/dark cycle in the Kobe University Life-Science Laboratory.
Before DIN administration and CUMS exposure, a period of 1 week was provided to acclimate the mice to the breeding environment with ad libitum access to a pellet diet (DC-8;
Clea Japan, Tokyo, Japan) and filtered water. This study was approved by the Institutional Animal Care and Use Committee (permission number: 26-05-07) and was carried out according to the
Kobe University Animal Experimental Regulations.

Male and female C57BL/6NCrSlc (B6N) mice embryos treated as normal phenotype and
B6N-XY^POS mice embryos [32 (link)] were used,
and all of their dams were maintained in 40.5 × 20.5 × 18.5-cm ventilated cages (Sealsafe
Plus Mouse, Tecniplast, Buguggiate, Italy) under controlled temperature (23 ± 2°C),
humidity (50 ± 10%) and ventilation (75 times/hr) on a 14-hr light/10-hr dark cycle at the Kobe University Life-Science Laboratory with
ad libitum access to water and a pellet diet (DC-8, Clea Japan, Tokyo,
Japan). This study was approved by the Institutional Animal Care and Use Committee
(Permission #25-06-03) and carried out according to the Kobe University Animal
Experimental Regulations.