Fitc texas red conjugated secondary antibodies

Paraffin tissue sections were stained following antigen retrieval with citrate buffer, pH 6.0, and blocking using 5% horse serum in PBS. Immunohistochemistry was performed with biotin-conjugated Ulex europaeus I (UEA)(1:100, Vector Laboratories). Peroxidase was quenched using 3% H₂O₂(Sigma) prior incubation with streptavidin-conjugated horseradish peroxidase (HRP) (1:200, Vector Laboratories) followed by diaminobenzidine (DAB) (Vector Laboratories). Samples were mounted with VectaMount (Vector Laboratories). Immunofluorescence was performed using UEA, Griffonia simplicifolia Isolectin B4 (IB4) (1:50 and 1:100, Vector Laboratories), and anti-αSMA (1:500, Sigma). FITC/Texas Red-conjugated secondary antibodies (1:200, Vector Laboratories) were used. Samples were mounted using Prolong Gold with DAPI. Images were acquired using Nikon C2 confocal microscope and NIS-Elements C imaging (Nikon).