Xenopus laevis oocytes (Dumont stage V or VI) were purchased (Ecocyte Bioscience US LLC, Austin, Texas) and handled as described (Maksaev & Haswell, 2015 (link)). Oocytes were injected with 50 nl of 1000 ng/μl of RNA the day after isolation. Fluorescent imaging of the oocytes was carried out 48–72 h after injection. Oocytes were mounted on concave slides and covered with coverslips. Confocal imaging of the periphery of the oocytes was performed using Olympus Fluoview 1000 with BX61 microscope and the Olympus FV10-ASW software suite.
Fluoview 1000 with bx61 microscope
Manufactured by Olympus
The Fluoview 1000 with BX61 microscope is a high-performance confocal laser scanning microscope system. It is designed for fluorescence imaging and analysis of biological samples. The system features a BX61 upright microscope, a laser scanning unit, and specialized software for image acquisition and processing.
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Lab products found in correlation
2 protocols using fluoview 1000 with bx61 microscope
1
Xenopus Oocyte Imaging Workflow
2
Fluorescence Imaging of Xenopus Oocytes
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Xenopus laevis oocytes (Dumont stage V or VI) were purchased (Ecocyte Bioscience US LLC, Austin, Texas) and handled as described (Maksaev & Haswell,2015 ). Oocytes were injected with 50 nl of 1000 ng/μl of RNA the day after isolation. Fluorescent imaging of the oocytes was carried out 48–72 h after injection. Oocytes were mounted on concave slides and covered with coverslips. Confocal imaging of the periphery of the oocytes was performed using Olympus Fluoview 1000 with BX61 microscope and the Olympus FV10‐ASW software suite.
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Xenopus laevis oocytes (Dumont stage V or VI) were purchased (Ecocyte Bioscience US LLC, Austin, Texas) and handled as described (Maksaev & Haswell,
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