Microdialysis was performed as described previously22 (link)46 (link). Briefly, the animals were anesthetized with sodium pentobarbital, and surgery was performed to implant a guide cannula (AG-4; EICOM, Kyoto, Japan) into the nucleus accumbens. On the following day, a dialysis probe (AI-4-1, 1-mm membrane length; EICOM) was inserted through the guide cannula and was perfused with artificial cerebrospinal fluid (148 mM NaCl, 2.7 mM KCl, 1.2 mM CaCl2, and 0.85 mM MgCl2) at a flow rate of 1 μl min–1. After an equilibration period of one hour, samples were collected every 20 minutes. After collection of at least five baseline fractions, mice were administered MAP (1 mg kg–1, i.p.), and 6 more fractions of samples were collected. The concentration of dopamine in the samples was measured by HPLC on a 4.6 by 30 mm PP-ODS column (EICOM) that was maintained at 25 °C and equipped with an electrochemical detection system (HTEC-500, EICOM) and PowerChrom (EICOM).
Pp ods column
Manufactured by Eicom
The PP-ODS column is a type of reversed-phase chromatography column used for liquid chromatography (LC) analysis. It is designed with a stationary phase composed of polypropylene material and octadecylsilane (ODS) bonded ligands. The column's core function is to facilitate the separation and analysis of a wide range of organic compounds in various sample matrices.
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Lab products found in correlation
2 protocols using pp ods column
1
Microdialysis of Dopamine in Mouse NAc
2
HPLC-based Neurotransmitter Quantification
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The collected microdialysate samples (10 μL) were separated by HPLC using a PP-ODS column (EICOM) and a mobile phase containing 0.1 mol/L phosphate buffer (pH 6.0), 1% methanol, 50 mg L−1 ethylenediamine tetraacetic acid disodium, and 500 mg L−1 sodium 1-decanesulfonate at a flow rate of 0.5 mL min−1. The peaks corresponding to 5-HT and dopamine were amperometrically detected using a graphite electrode set at 400 mV with an Ag/AgCl reference electrode (RE-100; EICOM). Online data acquisition was performed using PowerChrom software (Version 2.2; AD Instruments Pty Ltd., Nagoya, Aichi, Japan). Before performing the microdialysis, the retention time of the HPLC peak for 5-HT and dopamine was determined using a standard solution. The peak height (mV) of 5-HT and dopamine at each measurement was converted into a percentage of the average of the last 4 pre-drug baseline values (percentage of baseline).
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