Cayman eia kit

Induced sputum was collected pre- and post-shift by inhalation of isotonic (0.9%) saline aerosol, in both the follicular and the luteal phases of the menstrual cycle, placed at 4°C and immediately sent to the laboratory. After centrifugation, aliquots of the supernatant fluids were stored at -80°C until further analysis. Cyclic AMP (cAMP) and testosterone levels were measured using Enzyme Immunoassays (Cayman-EIA kit, Michigan, USA). Moreover, PDE activity was evaluated using a PDE assay kit (BPS Bioscience, San Diego, CA). The measurements (PFTs, PC20, and blood collection) were performed on day 1 ± 3 days of menstruation and 14 ± 3 days after the start of menstruation.

Testosterone levels were measured using Cayman (Ann Arbor, MI) EIA kit Cat# 582701 (RRID : AB_2895148); dihydrotestosterone levels were measured using CUSAbio (College Park, MD) kit Cat# CSB-E07879r; luteinizing hormone levels were measured using CUSAbio kit Cat# CSB-E12654r; estradiol levels were measured using CUSAbio kit Cat# CSB-E05110r; corticosterone levels were measured using CUSAbio kit Cat# CSB-E07014r for Figure 2 data and Cayman EIA kit Cat# 501320 (RRID : AB_2868564) for the remainder experiments; and aldosterone levels were measured using Cayman EIA Cat# 10004377 (RRID : AB_2895148). All samples were measured in duplicate according to each of the manufacturer’s instructions. Serum, plasma, or intratesticular fluid samples were diluted until controls fitted the middle of the standard curve used. The number of animals used for each experiment is noted in the corresponding figure legend.