Tetrahydrofurane

N,N′-bis(acryloyl) cystamine (BAC), glutathione (GSH), 2-methoxyethanol (MetCel), phosphate-buffered saline tablets, potassium persulfate (KPS; crystallized from water), poly(vinylpyrrolidone) K90 (PVP), methacryloyl chloride, 1-aminopropan-2-ol, 6-aminohexanoic acid, tert-butyl carbazatem, methyl-6-aminohexanoate, and hydrazine hydrate were purchased from Sigma Aldrich (St. Louis, MO, USA). Poly(vinyl alcohol) 25/140 (PVA) was obtained from Wacker Chemie (München, Germany). 2-Ethoxyethanol (EtCel) and CH₃OH were purchased from VWR (Radnor, PA, USA). Dichloromethane, NaOH, and Na₂CO₃ were purchased from Lachema (Brno, Czech Republic). Tetrahydrofurane was purchased from Lachner (Neratovice, Czech Republic). EDC.HCl was purchased from Gen Script (Piscataway, NJ, USA). For the A4F characterization, the water of MilliQ purity was prepared by laboratory water purification system Milli-Q^® IQ 7000 (Merck KGaA, Darmstadt, Germany), and sodium azide (NaN₃; ≥99%, Lach-Ner, Neratovice, Czech Republic) was used as an antibacterial agent.

Hyaluronic acid (HA) was obtained from Contipro a.s (Dolni Dobrouc, Czech Republic) Particularly, HA of weight-average molecular weight and polydispersity (in brackets) (Mw = 15,000 [PDI = 1.5], 110,000 g/mol [PDI = 1.7], and 240,000 g/mol, [PDI = 1.8], respectively). Tetrahydrofurane (THF, 99.5%), isopropanol (IPA, 99.7%) were purchased from Lach-Ner (Neratovice, Czech Republic). Benzoyl chloride (BC, 99%), triethylamine (TEA, 98%), 10-undecylenic acid (98%), N,N-Diisopropylethylamine (DIPEA), 2-Hydroxy-4′-(2-hydroxyethoxy)-2-methylpropiophenone (Irgacure 2959), ammonium persulphate (APS), N,N,N′,N′-Tetramethylethylenediamine (TEMED, ~99%) and 2,2-dimethoxyphenylacetophenone (DMPA, Irgacure 651), riboflavin (98%), undecanoic acid, formic acid, sodium hydroxide, coenzyme Q10, α-Tocopherol, and curcumin were purchased from Sigma-Aldrich (Prague, Czech Republic). Deuterium oxide (D₂O) was purchased from CortecNet (Voisins le Bretonneux, France). 4-(dimethylamino) pyridine (DMAP, 99.5%) was obtained from Merck (Prague, Czech Republic). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and ethidium homodimer were purchased from Life Technologies (Carlsbad, CA, USA).