Minimum essential medium alpha medium

Manufactured by Corning

Sourced in United States

Minimum Essential Medium Alpha Medium is a cell culture medium formulation developed by Corning. It provides a balanced salt solution and essential nutrients required for the in vitro maintenance and growth of a variety of cell types.

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Lab products found in correlation

Hepes, by Corning (1 mentions) Premium grade fbs, by Avantor (1 mentions) Roflumilast, by Cayman Chemical (1 mentions) Forskolin, by Cayman Chemical (1 mentions) Isoproterenol, by Cayman Chemical (1 mentions) Mk571, by Cayman Chemical (1 mentions) Rolipram, by Cayman Chemical (1 mentions) Ceefourin 1, by Abcam (1 mentions) Fetal bovine serum (fbs), by Lonza (1 mentions) Penicillin, by Lonza (1 mentions) Streptomycin, by Lonza (1 mentions)

Close spelling variants of this product

Alpha Minimum Essential Medium (6 citations)

2 protocols using minimum essential medium alpha medium

Culturing HBEC6-KT and Calu-3 Airway Cells

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The human bronchial epithelial cell (HBEC6-KT) line was cultured in Keratinocyte Serum-Free Medium 1X (Gibco) according to the manufacturers directions and supplemented with P/S (VWR) at 1X^{70 (link)}. Human airway epithelial cell line Calu-3 (ATCC HTB-55) were cultured in Minimum Essential Medium Alpha Medium (Corning) and supplemented with Premium Grade FBS (VWR) at 10%, HEPES (Corning) at 1X, and P/S (VWR) at 1X. The following chemical reagents were dissolved in DMSO, cAMP elevating agents Forskolin (Cayman Chemical) and Isoproterenol (Cayman Chemical), ABCC4 inhibitors MK-571 (Cayman Chemical) and Ceefourin 1 (Abcam), PDE inhibitors Roflumilast (Cayman Chemical) and Rolipram (Cayman Chemical), and CFTR Inhibitor (Selleck Chemicals).

Nguyen J.P., Bianca M., Huff R.D., Tiessen N., Inman M.D, & Hirota J.A. (2021). Modulation of cAMP metabolism for CFTR potentiation in human airway epithelial cells. Scientific Reports, 11, 904.

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Culturing Adipose-Derived Mesenchymal Stem Cells

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Human adipose-derived mesenchymal stem cells were purchased from Lonza (U.S.A.) and cultured in Minimum Essential Medium Alpha Medium (Corning, U.S.A.) containing 2 mM L-Glutamine and sodium pyruvate (Lonza, U.S.A) supplemented with 10% fetal bovine serum (FBS, Life Technologies, U.S.A.), 100 U/mL penicillin (Lonza, U.S.A.) and 100 μg/ml streptomycin (Lonza, U.S.A.) at 37 °C in 5% CO₂ atmosphere. All cellular experiments were conducted using adipose-derived mesenchymal stem cells of passage 6 or lower. Culture media without sodium pyruvate was used whenever hydrogen peroxide was involved.

Teo J.Y., Seo Y., Ko E., Leong J., Hong Y.T., Yang Y.Y, & Kong H. (2019). Surface Tethering of Stem Cells with H2O2-Responsive Anti-Oxidizing Colloidal Particles for Protection Against Oxidation-Induced Death. Biomaterials, 201, 1-15.

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