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Revert wash buffer

Manufactured by LI COR

The 1X LI-COR REVERT™ Wash Buffer is a laboratory reagent designed for use in various analytical procedures. It is a ready-to-use solution that serves as a washing buffer to facilitate the removal of unwanted substances from samples or assay plates. The buffer is formulated to maintain the appropriate pH and ionic conditions for effective washing, thereby supporting the overall integrity of the analytical process.

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2 protocols using revert wash buffer

1

Quantitative Total Protein Estimation

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Total protein standards were prepared by diluting 5 mg/mL bovine serum albumin (BSA) in DMD lysis buffer, and then diluting serially 1:2 six times (5–0.078 mg/mL). Test samples were thawed on ice. Aliquots were removed for total protein measurement. A total of 1 μL of each test sample or BSA standard was pipetted onto a nitrocellulose membrane, at least in duplicate measurement. After air drying, the membrane was incubated with 1X LI-COR REVERT stain (LI-COR, Lincoln, NE) as per the vendor protocol. The membrane was incubated in the stain for 5 min, and then washed twice in equal volumes of 1X LI-COR REVERT Wash Buffer. The dots on the membrane were visualized and quantitated on a LI-COR Odyssey CLX Imaging System (LI-COR, Lincoln, NE). Total protein on the samples was determined by interpolation of dot-blot fluorescence intensities into the BSA standard curve as described previously (36 (link), 37 (link)).
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2

Quantitative Total Protein Estimation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total protein standards were prepared by diluting 5 mg/mL bovine serum albumin (BSA) in DMD lysis buffer, and then diluting serially 1:2 six times (5–0.078 mg/mL). Test samples were thawed on ice. Aliquots were removed for total protein measurement. A total of 1 μL of each test sample or BSA standard was pipetted onto a nitrocellulose membrane, at least in duplicate measurement. After air drying, the membrane was incubated with 1X LI-COR REVERT stain (LI-COR, Lincoln, NE) as per the vendor protocol. The membrane was incubated in the stain for 5 min, and then washed twice in equal volumes of 1X LI-COR REVERT Wash Buffer. The dots on the membrane were visualized and quantitated on a LI-COR Odyssey CLX Imaging System (LI-COR, Lincoln, NE). Total protein on the samples was determined by interpolation of dot-blot fluorescence intensities into the BSA standard curve as described previously (36 (link), 37 (link)).
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