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3 4 5 dimethyl 2 thiazolyl 2 5 diphenyl 2h tetrazolium bromide mtt

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3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) is a chemical compound used in cell-based assays to measure cell viability and proliferation. It is a yellow tetrazolium salt that can be reduced to a purple formazan product by metabolically active cells. The intensity of the purple color can be quantified using a spectrophotometer, providing a measure of the number of viable cells in a sample.

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Lab products found in correlation

Methanol, by Merck Group (1 mentions) 2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (1 mentions) L glutamine, by Lonza (1 mentions) Fetal bovine serum (fbs), by HiMedia (1 mentions) Gallic acid, by Merck Group (1 mentions) Phosphate buffered saline (pbs), by Lonza (1 mentions) Ham s f12 with l gluta mine, by Lonza (1 mentions) Crystal violet, by Merck Group (1 mentions) Ldh activity assay, by Promega (1 mentions) Dulbecco s phosphate buffered saline dpbs, by Biowest (1 mentions) Ch3cn, by Scharlab (1 mentions) Human il 1β elisa set 2, by BD (1 mentions) Ch 2 cl 2, by Scharlab (1 mentions) Cell proliferation reagent wst 1, by Merck Group (1 mentions) Kh2po4, by Merck Group (1 mentions) Sodium chloride (nacl), by Merck Group (1 mentions) Pluronic f 127, by Merck Group (1 mentions) Na2hpo4, by Merck Group (1 mentions) Potassium chloride (kcl), by Merck Group (1 mentions) Penicillin streptomycin, by Biowest (1 mentions)

2 protocols using 3 4 5 dimethyl 2 thiazolyl 2 5 diphenyl 2h tetrazolium bromide mtt

1

Detailed Materials for Cell Culture Studies

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PCL in the form of pellets
(molecular weight 80,000), methanol (≥99.8% (GC) purity), DPPH,
and Folin–Ciocalteu reagent (Folin) were purchased from Sigma-Aldrich
Co. (USA). FA of 98/100% purity was obtained from Thermo Fisher Scientific
(USA). As for gallic acid, it was purchased from Merck Millipore Co.
(Germany), while the phosphate-buffered saline (PBS) was purchased
from Lonza, Belgium, and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) was obtained from SERVA Electrophoresis
GmbH (Germany). Sodium Carbonate was obtained from El-Nasr pharmaceutical
chemicals (Egypt) and Alizarin Red S Monohydrate was purchased from
MP Biomedicals, LLC (USA). The osteogenic media was prepared from
Dulbecco’s modified Eagle’s medium with 4.5 g/L glucose
and with l-glutamine and Ham’s F-12 with l-glutamine, purchased from BioWhittaker Lonza (USA), the fetal bovine
serum was obtained from HiMedia (Brazil), and the antib-anti (100×)
penicillin/streptomycin/amphotericin (antibiotic-antimycotic) was
obtained from Gibco Life Technologies Co., (USA). Finally, the β-glycerophosphate
disodium salt hydrate and dexamethasone (9α-fluoro-16α-methylprednisolone)
were obtained from AppliChem GmbH (Germany) with l-ascorbic
acid Na salt from SERVA Electrophoresis GmbH (Germany).
Sadek K.M., Mamdouh W., Habib S.I., El Deftar M, & Habib A.N. (2021). In Vitro Biological Evaluation of a Fabricated Polycaprolactone/Pomegranate Electrospun Scaffold for Bone Regeneration. ACS Omega, 6(50), 34447-34459.
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2

Synthesis and Characterization of Hexanuclear Molybdenum Clusters

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The chemical reagents tetraethylorthosilicate (TEOS), n-cetyltrimethylammonium bromide (CTABr), sodium hydroxide (NaOH), (3aminopropyl) triethoxysilane (APTES, 99 %), Pluronic® F-127, 9,10anthracenediyl-bis(methylene)dimalonic acid (ABDA, ≥90 %), as well as the reagents to prepare the phosphate-buffered saline (PBS) solution namely, RPMI 1460 media (R8758), lipopolysaccharide (LPS) from Escherichia coli O111:B4 (L2630-100MG), nigericin (NG) (N7143-5MG), Crystal Violet, Cell Proliferation Reagent WST-1, NaCl (≥99 %), KCl (≥99.0 %), Na 2 HPO 4 (≥99.0 %) and KH 2 PO 4 (≥99.5 %) were supplied by Sigma-Aldrich, 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) was acquired from Serva. LDH activity assay was purchased from Promega (G7891). Human IL-1β ELISA Set II was obtained from BD Biosciences (557953). Penicillin/streptomycin, Dulbecco's modified Eagle's medium (DMEM), minimum essential medium (MEM), fetal bovine serum (FBS) and Dulbecco's phosphate-buffered saline (DPBS), were supplied by Biowest. Solvents THF, CH 2 Cl 2 and CH 3 CN were acquired from Scharlab, S.L. Distilled water was used to prepare the aqueous solutions. Hexanuclear cluster (Bu 4 N) 2 [-Mo 6 I 8 (CH 3 CO 2 ) 6 ] (1) was synthesized according to the procedure reported in the literature [72] (link).
de la Torre C., Gavara R., García-Fernández A., Mikhaylov M., Sokolov M.N., Miravet J.F., Sancenón F., Martínez-Máñez R, & Galindo F. (2022). Enhancement of photoactivity and cellular uptake of (Bu4N)2[Mo6I8(CH3COO)6] complex by loading on porous MCM-41 support. Photodynamic studies as an anticancer agent. Biomaterials advances, 140.
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