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Recombinant lf rlf

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Recombinant LF (rLF) is a laboratory product produced by List Biological Laboratories. It is a recombinant form of the lethal factor (LF) protein. LF is a component of the anthrax toxin. The core function of rLF is to serve as a research tool for the study of anthrax toxin and related biological processes.

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Lab products found in correlation

Kingfisher 96 magnetic particle processor, by Thermo Fisher Scientific (2 mentions) Dynabeads myone tosyl activated magnetic beads, by Thermo Fisher Scientific (2 mentions) 4800 plus proteomics analyzer maldi tof ms, by AB Sciex (2 mentions) Gene amp pcr thermocycler, by Thermo Fisher Scientific (2 mentions)

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Recombinant LF (2 citations)

2 protocols using recombinant lf rlf

1

Anthrax Lethal Factor Detection Protocol

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Chemicals and reagents were purchased from Sigma-Aldrich (Saint Louis, MO) except where indicated. Dynabeads® MyOne™ Tosyl-activated magnetic beads were purchased from Invitrogen Co. (Carlsbad, CA). Recombinant LF (rLF) was purchased from List Biological Laboratories (Campbell, CA). Two non-neutralizing monoclonal anti-LF antibodies (LF-mAb) were prepared in the Division of Scientific Resources of CDC as previously described [19 (link)]. AIGIV was obtained from the Division of Strategic National Stockpile, CDC (Atlanta, GA). Pooled human donor plasma (n = 10 individuals per pool) (PH-plasma) and individual normal North American (NNA) donor serum and plasma samples were obtained from Interstate Blood Bank (Memphis, TN). Equipment included a Kingfisher 96 magnetic particle processor (Thermo Fisher Scientific., Waltham, MA), 4800 Plus Proteomics Analyzer (MALDI-TOF MS) (AB Sciex, Foster City, CA) and Gene Amp@ PCR thermocycler (ThermoFisher, Waltham, MA). All procedures and sample handling were conducted at Biosafety Level 2 containment as recommended and described [21 ]. Additional safety measures are described in each section as needed.
Gallegos-Candela M., Boyer A.E., Woolfitt A.R., Brumlow J., Lins R.C., Quinn C.P., Hoffmaster A.R., Meister G, & Barr J.R. (2017). Validated MALDI-TOF-MS method for anthrax lethal factor provides early diagnosis and evaluation of therapeutics. Analytical biochemistry, 543, 97-107.
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2

Anthrax Lethal Factor Detection Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Chemicals and reagents were purchased from Sigma-Aldrich (Saint Louis, MO) except where indicated. Dynabeads® MyOne™ Tosyl-activated magnetic beads were purchased from Invitrogen Co. (Carlsbad, CA). Recombinant LF (rLF) was purchased from List Biological Laboratories (Campbell, CA). Two non-neutralizing monoclonal anti-LF antibodies (LF-mAb) were prepared in the Division of Scientific Resources of CDC as previously described [19 (link)]. AIGIV was obtained from the Division of Strategic National Stockpile, CDC (Atlanta, GA). Pooled human donor plasma (n = 10 individuals per pool) (PH-plasma) and individual normal North American (NNA) donor serum and plasma samples were obtained from Interstate Blood Bank (Memphis, TN). Equipment included a Kingfisher 96 magnetic particle processor (Thermo Fisher Scientific., Waltham, MA), 4800 Plus Proteomics Analyzer (MALDI-TOF MS) (AB Sciex, Foster City, CA) and Gene Amp@ PCR thermocycler (ThermoFisher, Waltham, MA). All procedures and sample handling were conducted at Biosafety Level 2 containment as recommended and described [21 ]. Additional safety measures are described in each section as needed.
Gallegos-Candela M., Boyer A.E., Woolfitt A.R., Brumlow J., Lins R.C., Quinn C.P., Hoffmaster A.R., Meister G, & Barr J.R. (2017). Validated MALDI-TOF-MS method for anthrax lethal factor provides early diagnosis and evaluation of therapeutics. Analytical biochemistry, 543, 97-107.
+ Open protocol
+ Expand

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