Tumors and main organs (brain, heart, liver, spleen, lungs, and kidneys) collected from the mice were fixed in 4% paraformal-dehyde. To further evaluate the antitumor efficacy, paraffin-embedded tumor sections were stained for TUNEL apoptosis assay (Roche, Beijing, China) and immunohistochemistry assay of Ki67 (Santa Cruz Biotechnology, Santa Cruz, CA, USA) according to the manufacturer’s protocols. The cell apoptosis and proliferation of tumors were observed by optical microscopy. In addition, to assess biosafety, tissue sections of major organs were stained with H&E to observe histopathological changes.
Tunel apoptosis assay
Manufactured by Roche
Sourced in China
The TUNEL apoptosis assay is a laboratory technique used to detect and quantify programmed cell death, or apoptosis, in cells. The assay works by labeling the fragmented DNA that is characteristic of apoptotic cells, allowing for their identification and analysis.
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Lab products found in correlation
3 protocols using tunel apoptosis assay
1
Evaluating Antitumor Efficacy and Organ Safety
2
Apoptosis and Histopathology Assessment
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Tumors and main organs (brain, heart, liver, spleen, lungs, and kidneys) collected from the mice were fixed in 4% paraformaldehyde. Resected tumor tissue was stained for TUNEL apoptosis assay (Roche, Beijing, China) and immunohistochemistry assay of caspase3 (Santa Cruz Biotechnology, United States) according to the manufacturer’s protocols. Tissue sections of major organs were stained with H&E to observe histopathological changes.
3
Quantifying Apoptosis in Xenograft Tumors
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The extent of cell apoptosis in the tumor specimens from the subcutaneous xenograft models was evaluated using a TUNEL apoptosis assay according to the manufacturer’s instructions (Roche, Basel, Switzerland). For quantitative analysis, the TUNEL-positive cells that fulfilled the morphological criteria of apoptosis were counted in 5 randomly chosen fields (400 × magnification). The results are expressed as the mean percentage of apoptosis cells.
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