Pcr kit rr036a and rr420a

FG-4592 was purchased from Cayman Chemical Company (www.caymanchem.com), and normal saline (NS) was obtained from Changhai Hospital (Shanghai, China). The apoptosis detection kit was purchased from TransGen (Beijing, China). The PCR kit (RR036A and RR420A) was purchased from TAKARA (Japan). RPMI 1640, DMEM and fetal bovine serum (FBS) were supplied by Gibco (New York, USA). Organoid cultures were obtained from STEM CELL. BCL2, BAX, C-CASPASE3, GAPDH, NF-κB and P-IKK-β antibodies were supplied by CST. The TLR4 antibody was supplied by Proteintech. In Situ Cell Death Detection Kit was obtained from Roche (Basel, Switzerland). The primes were obtained from Shenggong Biotech (Shanghai, China). The list of primers is shown in Table 1.Table 1

qRT-PCR primers for the eight genes evaluated

Gene symbol	Forward	Reverse
TLR4	AAATGCACTGAGCTTTAGTGGT	TGGCACTCATAATGATGGCAC
IL-6	CTGCAAGAGACTTCCATCCAG	AGTGGTATAGACAGGTCTGTTGG
IGFBP2	CAGACGCTACGCTGCTATCC	CCCTCAGAGTGGTCGTCATCA
SOX2	GCGGAGTGGAAACTTTTGTCC	CGGGAAGCGTGTACTTATCCTT
REG2	CTGATGTTCCTGTCATACAGCC	CCAGGTCAAACGGTCTTCAATTA
REG3B	ACTCCCTGAAGAATATACCCTCC	CGCTATTGAGCACAGATACGAG
REG3D	GACTCCATGATCTGTCACTTGG	CATAGGGAAATGTTGGGTCACAA
HK1	CAAGAAATTACCCGTGGGATTCA	CAATGTTAGCGTCATAGTCCCC

APS was purchased from Shanghai Yuanye Biological Technology Company (Shanghai, China). Normal saline (NS) was obtained from ChangHai Hospital (Shanghai, China) and PBS buffer was purchased from Hyclone company (New York, USA). RPMI 1640 medium and fetal bovine serum (FBS) were provided by Gibco (New York, USA). IntestiCult™ Organoid Growth Medium (06005) and Matrigel (356231) were purchased from Stem Cell Technologies (Canada). FITC‐dextran was purchased from Sigma‐Aldrich (Saint Louis, USA). 2‐Methoxyestradiol (2‐MeOE2) was supplied by Selleck Chemicals (Houston, USA). The apoptosis detection kit (FA101) was purchased from Transgen (Beijing, China). The PCR kit (RR036A and RR420A) was purchased from TAKARA (Japan). The CCK8 kit (CK04) was obtained from Donjindo (Osaka, Japan). The cytometric bead array (CBA) (740446) was purchased from Biolegend (USA). BCL2, BAX, C‐CASPASE3, ZO‐1, Occludin, AKT1, OLFM4, Axin, Hif‐1α, β‐actin and GAPDH antibodies were supplied by Cell Signalling Technology (Massachusetts, USA). The primes were supplied by Shenggong Biotech (Shanghai, China). The list of primers is shown in Table 1.