P stat3 tyr

Manufactured by Cell Signaling Technology

Sourced in United States

P-STAT3 (Tyr) is an antibody that recognizes the phosphorylated form of STAT3 at tyrosine 705. STAT3 is a transcription factor that plays a key role in cellular signaling pathways.

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Lab products found in correlation

Pierce ecl, by Thermo Fisher Scientific (3 mentions) Pstat3 ser, by Cell Signaling Technology (2 mentions) P jnk, by Cell Signaling Technology (1 mentions) P erk, by Cell Signaling Technology (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Cell Signaling Technology (1 mentions) Prolong gold antifade reagent, by Cell Signaling Technology (1 mentions) Supersignal west dura substrate, by Thermo Fisher Scientific (1 mentions) Total stat3, by Cell Signaling Technology (1 mentions) Atp5a, by Abcam (1 mentions) β actin, by Cell Signaling Technology (1 mentions) Gapdh, by Abcam (1 mentions)

Close spelling variants of this product

Anti-p-STAT3 (tyr-705) antibody P-Tyr P-STAT3 STAT3

3 protocols using p stat3 tyr

Apoptosis Signaling Pathway Profiling

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ILL (cat. no. ALB-RS-6003) was purchased from ALB Technology Limited (Henderson, NV, USA), prepared as a 100 µM stock solution in dimethyl sulfoxide (Sigma Chemical Company, St. Louis, MO, USA), aliquoted, and stored at −80 °C before use. Primary antibodies against all cleaved caspases, cleaved poly (adenosine diphosphate-ribose) polymerase (PARP), Bax, SOD1, Akt, mitogen-activated protein kinase (ERK), C-Jun N-terminal kinase (JNK), phosphorylated (p)-AKT, p-ERK, p-JNK, STAT3, p-STAT3 (Ser), and p-STAT3 (Tyr) were purchased from Cell Signaling Technology (Danvers, MA, USA), while antibodies against Bcl-2, Bcl-xL, and SOD2 were obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA), and antibodies against survivin were acquired from Novus Biologicals LLC (Centennial, CO, USA). All secondary antibodies were obtained from Thermo Fisher Scientific (Waltham, MA, USA).

Rajina S., Kim W.J., Shim J.H., Chun K.S., Joo S.H., Shin H.K., Lee S.Y, & Choi J.S. (2020). Isolinderalactone Induces Cell Death via Mitochondrial Superoxide- and STAT3-Mediated Pathways in Human Ovarian Cancer Cells. International Journal of Molecular Sciences, 21(20), 7530.

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Physalin-Induced Modulation of pYSTAT3 Levels

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H1975 cells were treated with 15 μg/mL physalins for 6 h followed by treatment with recombinant IL-6 protein (25 ng/mL) to induce pYSTAT3 levels, fixed in 4% paraformaldehyde for 15 min on ice and permeabilized with precooled methanol for another 10 min on ice. Next, the cells were blocked with blocking solution (5% normal goat serum, 0.3% Triton X-100 in PBS) for 60 mins at room temperature. After aspirating the blocking solution, the diluted primary antibody p-STAT3-Tyr (1:500, Cell Signaling Technology) was applied and incubated overnight at 4°C. The next day, the slides were rinsed in PBS 3 times and incubated with a fluorochrome-conjugated secondary antibody diluted in antibody dilution buffer (1% BSA and 0.3% Triton X-100 in PBS) for 1–2 h away from the light. The slides were rinsed with PBS 3 times and coverslipped with Prolong^® Gold Anti-Fade Reagent (#9071; Cell Signaling) with DAPI (#8961; Cell Signaling).

Fu Y., Zhu F., Ma Z., Lv B., Wang X., Dai C., Ma X., Liu P., Lv H., Chen X., Chen Z, & Shen L. (2021). Physalis alkekengi var. franchetii Extracts Exert Antitumor Effects on Non-Small Cell Lung Cancer and Multiple Myeloma by Inhibiting STAT3 Signaling. OncoTargets and therapy, 14, 301-314.

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Western Blot Analysis of STAT3 Signaling

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Cultures treated with OSM were lysed in buffer containing the following: 150 mM NaCl, 10 mM Na₂HPO₄ (pH 7.2), 0.5% sodium deoxycholate, 1% NP-40, and protease inhibitor mixture. Forty μg of total cell lysate was separated by electrophoresis on 8% SDS-polyacrylamide gels and blotted with antibodies against P-STAT3 Tyr, P-STAT3 Ser, total STAT3, β-actin (Cell Signaling Technology; Beverly, MA), GAPDH, and ATP5A (Abcam; Cambridge, MA) (Park et al., 2014 (link); Park et al., 2015 (link); Park et al., 2012 (link)). Immunoreactivity was assessed using Pierce ECL® or SuperSignal® West Dura substrate (Thermo Scientific, Rockford, IL). For quantitative analyses, the densities of bands on immunoblots were measured with ImageJ software. For in vivo studies, spinal cord tissues were obtained from areas 5 mm rostral or caudal to the injury at the designated time point after SCI. The tissues were homogenized in ice-cold lysis buffer and equal amounts of protein (40 μg) were separated by SDS-PAGE. The blots were then incubated with antibodies and developed as described previously (Park et al., 2014 (link); Park et al., 2015 (link)).

Park K.W., Lin C.Y., Benveniste E.N, & Lee Y.S. (2016). Mitochondrial STAT3 is negatively regulated by SOCS3 and upregulated after spinal cord injury. Experimental neurology, 284(Pt A), 98-105.

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