Cd11c apc fire 750

Manufactured by BioLegend

Sourced in United States

CD11c APC Fire 750 is a fluorescent dye-conjugated antibody that binds to the CD11c cell surface marker. CD11c is expressed on the surface of dendritic cells, a type of antigen-presenting immune cell. This product can be used in flow cytometry applications to identify and quantify CD11c-positive cell populations.

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Lab products found in correlation

Cd11b bv421, by BioLegend (2 mentions) Cd86 apc, by Thermo Fisher Scientific (2 mentions) Cd40 pe cy7, by BioLegend (2 mentions) Dxp10, by Cytek (2 mentions) Cd80 pe, by BD (2 mentions) Annexin 5 pe, by BD (2 mentions) 7 aminoactinomycin d, by BD (1 mentions) 7 aad, by BD (1 mentions)

Close spelling variants of this product

CD11c-APC (71 citations) APC/Fire 750 (7 citations)

2 protocols using cd11c apc fire 750

Assessing BMDC Activation and Viability

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To assess BMDC activation status and viability after CS treatment, BMDCs were treated for 24 h. After treatment, cells were harvested and stained for CD11b BV421 (BioLegend, clone: M1/70), CD11c APC Fire 750 (BioLegend, clone: N418), I‐A/I‐E major histocompatibility complex (MHC) Class II V500 (BD Biosciences, clone: M5/114.15.2), CD40 PE‐Cy7 (BioLegend, clone: 3/23), CD80 PE (BD Pharmingen, clone: 16‐10‐A1), CD86 APC (eBiosciences, clone: GL1), Annexin V PE (BD Biosciences), and 7‐aminoactinomycin D (7AAD; BD Biosciences). Samples were analyzed on a Cytek DxP10 (Cytek Biosciences, Inc.) flow at the University of Nebraska‐Lincoln Flow Cytometry Service Center. Data were analyzed using FlowJo software (Becton, Dickinson and Company).

Lampe A.T., Farris E.J., Brown D.M, & Pannier A.K. (2020). High‐ and low‐molecular‐weight chitosan act as adjuvants during single‐dose influenza A virus protein vaccination through distinct mechanisms. Biotechnology and Bioengineering, 118(3), 1224-1243.

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Assessing BMDC Activation and Viability After CS Treatment

Check if the same lab product or an alternative is used in the 5 most similar protocols

To assess BMDC activation status and viability after CS treatment, BMDCs were treated for 24 h. After treatment, cells were harvested and stained for CD11b BV421 (BioLegend, San Diego, CA, USA, clone: M1/70), CD11c APC Fire 750 (BioLegend, Clone: N418), I-A/I-E MHC Class II V500 (BD Biosciences, San Jose, CA, USA, clone: M5/114.15.2), CD40 PE-Cy7 (BioLegend, clone: 3/23), CD80 PE (BD Pharmingen, San Jose, CA, USA, clone: 16–10-A1), CD86 APC (eBiosciences, San Diego, CA, USA, clone: GL1), Annexin V PE (BD Biosciences), and 7AAD (BD Biosciences). Samples were analyzed on a Cytek DxP10 (Cytek Biosciences, Inc., Fremont, CA, USA) flow at the University of Nebraska-Lincoln Flow Cytometry Service Center. Data was analyzed using FlowJo software (Becton, Dickinson and Company, Franklin Lakes, NJ, USA).

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