All procedures were approved by the Rockefeller University Institutional Animal Care and Use Committee. All mice were housed with a 12-hour light/12-hour dark cycle and fed ad libitum. Transgenic mouse lines (2xTg: APPswe/PSEN1dE9; 1xTg: Tg2576 harboring HuAPP695.KM670/671NL; 3xTg: PS1M146V, APPswe and tauP301L], and C57BL/6J WT mice were obtained from The Jackson Laboratory (Bar Harbor, ME, USA). Mice were anesthetized via Nembutal IP injection (50-100 µl) and intracardiac perfusion was performed first with 20 ml ice cold PBS then 20 ml ice cold PBS with 4% paraformaldehyde. Dissected cerebral tissue was post-fixed overnight in PBS with 4% PFA at 4°C followed by 1 hour at room temperature.
Tg2576
Manufactured by Jackson ImmunoResearch
Sourced in United States
Tg2576 is a transgenic mouse model that overexpresses the 695-amino acid isoform of the human amyloid precursor protein (APP) with the Swedish double mutation (K670N/M671L). This model is commonly used in Alzheimer's disease research to study the pathological features of the disease.
Automatically generated - may contain errors
Lab products found in correlation
Appswe psen1de9, by Jackson ImmunoResearch (1 mentions)
Appswe, by Jackson ImmunoResearch (1 mentions)
Ps1m146v, by Jackson ImmunoResearch (1 mentions)
Km670 671nl, by Jackson ImmunoResearch (1 mentions)
Taup301l, by Jackson ImmunoResearch (1 mentions)
C57bl 6j wt, by Jackson ImmunoResearch (1 mentions)
2 protocols using tg2576
1
Transgenic Alzheimer's Mouse Model
2
Transgenic AD Mouse Model
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Transgenic mice (Tg-2576) containing the K670N/M671L (AβPP) Swedish double mutation, which leads to familial early onset AD [37 (link)], were obtained from The Jackson Laboratory (Bar Harbor, ME, U.S.A.) and maintained at the University of Tennessee, Memphis animal facility after experimental approval by the Institutional Animal Care and Use Committee of the University of Tennessee.
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