Microbiological media (
tryptone soy broth; TSB, and
tryptone soy agar; TSA) were purchased from Hi-Media, India.
Peptone and
sodium chloride were purchased from Oxoid, UK.
Hydrochloric acid,
ethyl acetate, and
sulfuric acid were purchased from Honeywell™, Charlotte, NC, USA.
l-rhamnose was purchased from Sigma-Aldrich, Darmstadt, Germany. Orcinol was obtained from SDFCL, Chennai, India.
Carbopol gel,
phosphate-buffered saline (PBS) tablets, and
absolute ethyl alcohol were purchased from Merck, Darmstadt, Germany. All chemicals and reagents were of analytical grade.
Two hospital-acquired human pathogenic bacteria—
Acinetobacter baumannii and
Staphylococcus aureus—were collected from a clinical setting in Cairo, Egypt. They were identified as multidrug-resistant bacteria.
The adult human dermal fibroblast (HDFa) cell line (PCS-201-012, HDFa) was purchased from the American Type Culture Collection (ATCC).
EMEM medium, fetal bovine serum (FBS), non-essential amino acids (NEAAs), penicillin, and
streptomycin were supplied by Thermo Fisher (Hessen, Germany).
MTT reagent was supplied by Thermo Fisher, Germany. All other materials were purchased from Sigma-Aldrich and used as supplied.
Abo-zeid Y., Bakkar M.R., Elkhouly G.E., Raya N.R, & Zaafar D. (2022). Rhamnolipid Nano-Micelles versus Alcohol-Based Hand Sanitizer: A Comparative Study for Antibacterial Activity against Hospital-Acquired Infections and Toxicity Concerns. Antibiotics, 11(5), 605.