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2 protocols using carbopol gel

1

Antibacterial and Cytotoxicity Evaluation

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Microbiological media (tryptone soy broth; TSB, and tryptone soy agar; TSA) were purchased from Hi-Media, India. Peptone and sodium chloride were purchased from Oxoid, UK. Hydrochloric acid, ethyl acetate, and sulfuric acid were purchased from Honeywell™, Charlotte, NC, USA. l-rhamnose was purchased from Sigma-Aldrich, Darmstadt, Germany. Orcinol was obtained from SDFCL, Chennai, India. Carbopol gel, phosphate-buffered saline (PBS) tablets, and absolute ethyl alcohol were purchased from Merck, Darmstadt, Germany. All chemicals and reagents were of analytical grade.
Two hospital-acquired human pathogenic bacteria—Acinetobacter baumannii and Staphylococcus aureus—were collected from a clinical setting in Cairo, Egypt. They were identified as multidrug-resistant bacteria.
The adult human dermal fibroblast (HDFa) cell line (PCS-201-012, HDFa) was purchased from the American Type Culture Collection (ATCC). EMEM medium, fetal bovine serum (FBS), non-essential amino acids (NEAAs), penicillin, and streptomycin were supplied by Thermo Fisher (Hessen, Germany). MTT reagent was supplied by Thermo Fisher, Germany. All other materials were purchased from Sigma-Aldrich and used as supplied.
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2

Cell culture protocol for Vero cells

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Microbiological media (tryptone soy broth; TSB, and tryptone soy agar; TSA) were purchased from Hi-Media, Mumbai, India. Peptone and sodium chloride were purchased from Oxoid, UK. Hydrochloric acid, ethyl acetate, and sulfuric acid were purchased from Honeywell™, Charlotte, NC, USA. l-rhamnose was purchased from Sigma-Aldrich, Darmstadt, Germany. Orcinol was obtained from SDFCL, Chennai, India. Carbopol gel, phosphate-buffered saline (PBS) tablets, and absolute ethyl alcohol were purchased from Merck, Darmstadt, Germany. All chemicals and reagents were of analytical grade.
The African green monkey kidney epithelial cell line (Vero) was purchased from the American Type Culture Collection (ATCC). Vero cells were cultured in complete Dulbeccos modified eagle medium (cDMEM), containing 10%, fetal bovine serum (FBS), 1% non-essential amino acids (NEAAs), 1% penicillin, and streptomycin and 1% l-glutamine, purchased from Thermo Fisher Scientific, Waltham, MA, USA.
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