The sections were analyzed with the microscopes: Olympus BX51, Olympus FV 1200 and Leica sp-2 AOBS confocal. The figure preparation was done with Adobe Photoshop CS6 (Adobe Systems, San Jose, CA) and Canvas X (ACD Systems, Canada).
Sp 2 aobs confocal
Manufactured by Leica
The Sp-2 AOBS confocal is a high-performance laser scanning microscope system designed for advanced imaging applications. It features an Acousto-Optical Beam Splitter (AOBS) that allows for rapid and precise control of the excitation laser wavelengths, enabling flexible and efficient use of fluorophores. The system provides exceptional image quality and resolution, making it a versatile tool for a wide range of research and analytical applications.
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Lab products found in correlation
2 protocols using sp 2 aobs confocal
1
Microscopic Imaging of Cellular Structures
2
Fluorescence Microscopy Image Analysis
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The analysis of the sections was made with a fluorescence microscope (Olympus BX51) equipped with appropriate filter combinations. Photomicrographs were obtained with a digital camera (Olympus DP72) adapted to the microscope. Additionally, sections were also analyzed with an Olympus FV 1200 or Leica sp-2 AOBS confocal microscope to assess the actual coexpression of two different markers in the same cell. Final adjustment of contrast and brightness of the photomicrographs was made with Adobe PhotoShop CS4 (Adobe Systems, San Jose, CA, USA). The compositions of the figures were mounted on plates using Canvas 11 (ACS Systems International, Santa Clara, CA, USA).
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