Heleos multi angle laser light scattering

Samples were separated over a WTC-0200S (Wyatt Technologies) size exclusion column utilizing an Agilent 1200 HPLC system at a flow rate 0.7 ml/min of MALS buffer (50 mM phosphate, 100 mM NaCl and 200 ppm NaN₃, pH 7.2). Sample volume of 25 µL per injection was loaded onto the column, and the column was run for 35 min. Eluted peaks were detected using a UV-Vis detector (Agilent), a Wyatt HELEOS multi-angle laser light scattering (MALS) detector, and an Optilab rEX differential refractometer (Wyatt Technology Corporation). The number-average particle molecular weight, Mn, was measured across FWHM of each peak with Astra 5.3.14 software (Wyatt Technology Corporation) using a previously calculated dn/dc value of 0.185 mL/g.

Samples were separated over a WTC-0200S (Wyatt Technologies) size exclusion column utilizing an Agilent 1200 HPLC system at a flow rate 0.7 ml min⁻¹ of MALS buffer (50 mM phosphate, 100 mM NaCl and 200 ppm NaN₃, pH 7.2). Sample volume of 25 μL per injection was loaded onto the column, and the column was run for 35 min. Eluted peaks were detected using a UV-Vis detector (Agilent), a Wyatt HELEOS multi-angle laser light scattering (MALS) detector, and an Optilab rEX differential refractometer (Wyatt Technology Corporation). The number-average particle molecular weight, Mn, was measured across FWHM of each peak with Astra 6.0.3.16 (Wyatt Technology Corporation) using a previously calculated dn/dc value of 0.185 mL g⁻¹.