Modified masson s trichrome staining kit

The sections were immersed in a staining solution and incubated overnight at 37°C. Next, the sections were stained by adding a solution of aniline blue for 2-3 minutes. Subsequently, Mayer’s hematoxylin solution was added to the sections for a staining duration of 2-3 minutes. Then, the sections were briefly treated with an acid differentiation solution, followed by rinsing with tap water for 10 minutes. Next, a solution of Ponceau acid fuchsin was added to the sections for a staining duration of 10 minutes. The sections were treated with a solution of phosphomolybdic acid for 10 minutes. The remaining dye solution was discarded, and the sections were directly stained with aniline blue for 5 minutes without rinsing. An acetic acid working solution was prepared by mixing acetic acid and water in a 1:2 ratio, and the sections were rinsed in this solution for 2 minutes. Finally, the sections were cleared with xylene for 1-2 minutes each time. Ultimately, the sections were mounted using neutral resin. The main reagents used in the above steps were from the Modified Masson’s Trichrome Staining Kit (Solarbio, G1346).