Memantine hydrochloride and the NETosis assay kit were purchased from Cayman Chemical (Ann Arbor, MI). The NET activator PMA, phagocytosis inhibitor Cytochalasin D, MPO antibody, and a S100A9 antibody were purchased from Abcam (Cambridge, MA). The neutrophil elastase (NE) ELISA Kit was purchased from R&D Systems (Minneapolis, MN). DNA fluorescent dye Picogreen was purchased from Thermo Fisher Scientific (Waltham, MA), CHRNA7 (α7R encoding gene), and S100A9 siRNA (small interfering RNA) kits were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). The CHRNA7 antibody was purchased from GenScript (Piscataway, NJ). The S100A9 ELISA kit was purchased from CUSABIO (Wuhan, China).
S100a9 antibody
Manufactured by Abcam
S100A9 antibody is a protein that binds to and detects the presence of the S100A9 protein, which is involved in the regulation of inflammatory processes and other cellular functions. This antibody can be used in various laboratory techniques to identify and quantify the S100A9 protein in samples.
Automatically generated - may contain errors
Lab products found in correlation
Netosis assay kit, by Cayman Chemical (1 mentions)
Phorbol myristate acetate (pma), by Abcam (1 mentions)
Memantine hydrochloride, by Cayman Chemical (1 mentions)
Mpo antibody, by Abcam (1 mentions)
Ne elisa kit, by R&D Systems (1 mentions)
Cytochalasin d, by Abcam (1 mentions)
Polyvinylidene fluoride membrane, by Merck Group (1 mentions)
2 protocols using s100a9 antibody
1
Neutrophil Extracellular Trap Assay
2
Quantitative Analysis of Calprotectin Proteins
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Protein extracts of mouse lung tissue were collected as previously described.17 (link) Proteins were separated by 10% sodium dodecyl-sulfate polyacrylamide gel electrophoresis and transferred onto polyvinylidene fluoride membranes (Millipore, Burlington, MA, USA). The membranes were blocked in Tris-buffered saline containing 5% skimmed milk and 0.1% Tween-20 for 1 hour at room temperature before incubating with the rabbit anti-calprotectin antibody (1:500; MyBioSource, San Diego, CA, USA) and S100A8 antibody (1:1,000; Abnova, Taipei, Taiwan), S100A9 antibody (1:1,000; Abcam, Cambridge, UK) overnight at 4°C. The membranes were then incubated with an horseradish peroxidase-conjugated secondary antibody for 1 hour at room temperature. Detection was performed using the enhanced chemiluminescence plus Western Blot Detection System (ATTO Corporation, Tokyo, Japan) on X-ray film. The relative protein abundance normalized to β-actin was determined by quantitative densitometry (Sigma-Aldrich).
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