Ab32572

Manufactured by Cell Signaling Technology

Sourced in United States

Ab32572 is a primary antibody product offered by Cell Signaling Technology. It is a research-use tool designed for the detection of specific protein targets in various experimental applications.

Automatically generated - may contain errors

Lab products found in correlation

Immobilon ecl ultra western hrp substrate, by Merck Group (1 mentions) Chemidoc mp imaging system, by Bio-Rad (1 mentions) Anti gapdh, by Abcam (1 mentions) Ab38113, by Abcam (1 mentions) Anti myc, by Cell Signaling Technology (1 mentions) Anti β actin, by Merck Group (1 mentions) Ab92547, by Cell Signaling Technology (1 mentions) Ab198288, by Abcam (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions)

3 protocols using ab32572

Western Blotting Analysis of Signaling Proteins

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A standard protocol was used for Western blotting using primary antibodies
specific for β-catenin (ab32572), TAZ (4883S), p-YAP (13008T), p-GSK-3β (5558),
p-β-catenin (2009), p-MOB1 (D2F10), and p-LATS1 (9157) from Cell Signaling
Technology (Danvers, MA, USA), as well as anti-GAPDH from Abcam (Shanghai,
China). Immobilon ECL Ultra Western HRP Substrate (Millipore, Bedford, MA, USA)
was used to develop blots, which were imaged with a ChemiDoc MP Imaging System
(Bio-Rad, Shanghai, China).

Xiao Y., Liu Q., Peng N., Li Y., Qiu D., Yang T., Kang R., Usmani A., Amadasu E., Borlongan C.V, & Yu G. (2022). Lovastatin Inhibits RhoA to Suppress Canonical Wnt/β-Catenin Signaling and Alternative Wnt-YAP/TAZ Signaling in Colon Cancer. Cell Transplantation, 31, 09636897221075749.

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Western Blotting for Protein Expression

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Western blotting was performed as described previously 52 (link). Briefly, 50 microgram of total protein was loaded for each sample. Primary antibody incubation was performed at 4 °C overnight with gentle shaking, and secondary antibody was incubated with membrane at room temperature for 1 h. Antibodies used in this study were anti-KDM6A (Bethyl Lab, A302-374A, 1:4000), anti-KDM6B (Abcam, ab38113, 1:1000), anti-β-Catenin (Abcam, ab32572, 1:2000), anti-MYC (Cell Signaling Technology, 18583, 1:1000), anti-AXIN2 (Proteintech, 20540-1-AP, 1:2000), and anti-β-actin (Sigma, A2228, 1:5000).

Zhang J., Ying Y., Li M., Wang M., Huang X., Jia M., Zeng J., Ma C., Zhang Y., Li C., Wang X, & Shu X.S. (2020). Targeted inhibition of KDM6 histone demethylases eradicates tumor-initiating cells via enhancer reprogramming in colorectal cancer. Theranostics, 10(22), 10016-10030.

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Protein Expression Analysis in RCC Tissues

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Cells or RCC tissues were lysed in RIPA on ice for 15 min, and the mixture was centrifuged for isolating protein samples. The concentration of protein was determined by BCA method. After adjusting protein samples to the same concentration (2ug/ul), they were denaturated, separated by SDS-PAGE, and loaded on PVDF membrane. The membrane was cut into small pieces according to the molecular size and blocked in TBST containing 5% skim milk for 2 h. They were incubated with primary (1:1000) and secondary antibodies (1:3000), followed by band exposure using ECL and grey value analyses using ImageJ software. Antibodies were purchased from Abcam (APOC1: ab198288, Wnt3a: ab219412, β-Catenin: ab32572, Vimentin: ab92547, N-cadherin: ab76011) and Cell Signaling Technology (GAPDH: 5174, MMP2: 40994, MMP9: 13667, CCND1: 55506, TCF7: 2203, anti-rabbit and anti-mouse secondary antibodies).

jiang h., Tang J., Xue D., Chen Y., Wu T., Zhuang Q., & He X. (2020). Apolipoprotein C1 stimulates the malignant process of renal cell carcinoma via the Wnt3a signaling.

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