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The A21088 is a laboratory instrument designed for performing spectrophotometric analysis. It is capable of measuring the absorbance of light by samples across a range of wavelengths, allowing for the quantification of various analytes.

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Lab products found in correlation

A10043, by LI COR (2 mentions) Sigma sab2502119, by Merck Group (2 mentions) Trizol kit, by Thermo Fisher Scientific (2 mentions) Laemmli sample buffer, by Bio-Rad (2 mentions) Tris glycine sds page, by Bio-Rad (2 mentions)

2 protocols using a21088

1

Quantifying Protein Expression in Ischemic Muscle and BMDMs

Check if the same lab product or an alternative is used in the 5 most similar protocols
Proteins from ischemic muscle tissue (100–150 mg) were extracted using Trizol kit (Fisher 15596026). Proteins from BMDMs (1.5million/mL) were collected by using Laemmli sample buffer (Bio-Rad, cat.1610737) containing 2-βME; then boiled at 95°C and aliquoted. Aliquots from tissue lysates or cell lysates were separated by Tris/Glycine/SDS–PAGE (Bio-Rad 1610732) and blotted into PVDF membranes. Membranes were blocked in fluorescent blocking buffer (Rockland Immunochemicals, cat.MB070) and probed at 4°C overnight with antibodies against VEGF-A (1:1000; Sigma SAB2502119); cleaved mature IL-1β (1:500; Cell Signaling Technology 52718); β-Actin (1:1000, sc-47778). After multiple washes, membranes were incubated with fluorescent secondary antibodies (Thermo Fisher A21088, 1:5000; Thermo Fisher A10043, 1:5000; and LI-COR 926–32212, 1:5000). Protein bands were quantified by densitometry, using the LI-COR Imager (Odyssey CLx) and normalization to β-Actin.
Mantsounga C.S., Lee C., Neverson J., Sharma S., Healy A., Berus J.M., Parry C., Ceneri N.M., López-Giráldez F., Chun H.J., Lu Q., Sellke F., Choudhary G, & Morrison A.R. (2022). Macrophage IL-1β promotes arteriogenesis by autocrine STAT3- and NF-κB-mediated transcription of pro-angiogenic VEGF-A. Cell reports, 38(5), 110309.
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2

Quantifying Protein Expression in Ischemic Muscle and BMDMs

Check if the same lab product or an alternative is used in the 5 most similar protocols
Proteins from ischemic muscle tissue (100–150 mg) were extracted using Trizol kit (Fisher 15596026). Proteins from BMDMs (1.5million/mL) were collected by using Laemmli sample buffer (Bio-Rad, cat.1610737) containing 2-βME; then boiled at 95°C and aliquoted. Aliquots from tissue lysates or cell lysates were separated by Tris/Glycine/SDS–PAGE (Bio-Rad 1610732) and blotted into PVDF membranes. Membranes were blocked in fluorescent blocking buffer (Rockland Immunochemicals, cat.MB070) and probed at 4°C overnight with antibodies against VEGF-A (1:1000; Sigma SAB2502119); cleaved mature IL-1β (1:500; Cell Signaling Technology 52718); β-Actin (1:1000, sc-47778). After multiple washes, membranes were incubated with fluorescent secondary antibodies (Thermo Fisher A21088, 1:5000; Thermo Fisher A10043, 1:5000; and LI-COR 926–32212, 1:5000). Protein bands were quantified by densitometry, using the LI-COR Imager (Odyssey CLx) and normalization to β-Actin.
Mantsounga C.S., Lee C., Neverson J., Sharma S., Healy A., Berus J.M., Parry C., Ceneri N.M., López-Giráldez F., Chun H.J., Lu Q., Sellke F., Choudhary G, & Morrison A.R. (2022). Macrophage IL-1β promotes arteriogenesis by autocrine STAT3- and NF-κB-mediated transcription of pro-angiogenic VEGF-A. Cell reports, 38(5), 110309.
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