Tracegold tg 5silms capillary column

¹³C-labeling pattern of proteinogenic amino acids were determined by gas chromatography coupled with mass spectrometry (GC-MS) as described in Schmitz et al. (2015a ). A Trace GC Ultra coupled to an ISQ single quadrupole mass spectrometer with a TriPlus RSH autosampler (all Thermo Fisher Scientific, Waltham, MA, USA) was used. For separation, a TraceGOLD TG-5SilMS capillary column (length: 30 mm, inner diameter: 0.25 mm, film thickness: 0.25 μm, Thermo Fisher Scientific, Waltham, MA, USA) was used. A sample volume of 2 μL was injected at 270°C with a split ratio of 1:50. The flow rate of the carrier gas helium was set to 1 mL min⁻¹ and the oven temperature was kept constant for 1 min at 140°C. The temperature was increased with a gradient of 10°C min⁻¹ to 310°C and then kept constant for 1 min. The temperature of the transfer line and the ion source were both set to 280°C. Ionization was performed by electron impact ionization at −70 eV. All raw data were analyzed using Xcalibur (Thermo Fisher Scientific, Waltham, MA, USA).

Gas chromatography-mass spectrometry (GC/MS) analyses were performed on a Thermo Scientific (Waltham, MA, USA) Trace 1310 GC coupled with a Thermo Scientific ISQ7000 mass detector and equipped with a Thermo Scientific Trace Gold TG-5SILMS capillary column (30 mm, 0.25 mm inner diameter, 0.25 µm film thickness). The oven temperature program was initiated at 50 °C and held for 1 min before raising the temperature 3 °C/min to 300 °C, then holding for 10 min. He (99.9999%) was used as the carrier gas with a flow rate of 2.2 ml/min. The injector temperature was 250 °C with a split ratio of 1/50. Mass spectra were recorded at 70 eV with a mass range from m/z 33 to 550. Constituents were identified and declared if they represented at least 0.1% of the total volume of the plant oil. Constituents of each plant oil are featured in Additional file 1: Table S1.