Dextrose

Thymoquinone, ocimene, and amphotericin B, foetal bovine serum, glutamine, and antibiotics (penicillin G, 60 mg/L; streptomycin, 100 mg/L; standard amphotericin B, 50 μL/L) were purchased from Sigma-Aldrich (Irvine, UK). Miramistin was purchased from Carbosynth Ltd. (Compton, UK). Dimethyl sulfoxide (DMSO) reagent grade, sodium hydroxide, disodium phosphate, and citric acid were purchased from Sigma-Aldrich (Irvine, UK). Dextrose, bacteriological peptone, yeast extract, agar number 2, Sabouraud Dextrose agar (SDA), malt extract agar (MEA), Hestrin and Schramm agar (HSA), Hestrin and Schramm media (HS), and Ringer’s solution were purchased from Lab M (Bury, UK). RPMI-1640 (Roswell Park Memorial Institute) was purchased from Fisher Scientific (Cramlington, UK). All media and reagents were prepared according to the manufacturer’s instructions.

Phosphate buffered saline (PBS, pH7.4) was purchased from Sigma-Aldrich (Gillingham, UK). Dimethyl sulfoxide (DMSO) HPLC grade, sodium hydroxide, disodium phosphate, citric acid and acetone were purchased from Sigma-Aldrich (Irvine, UK). Dextrose, bacteriological peptone, yeast extract, Hestrin and Schramm agar (HSA), Hestrin and Schramm media (HS) were purchased from Lab M (Bury, UK). HPLC gradient grade water, methanol, poly(ethylene glycol) (PEG400), and trifluoroacetic acid (TFA) was purchased from Fisher Scientific (Cramlington, UK). All media and reagents were prepared according to the manufacturer’s instructions.