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Ai14 tdtf f reporter

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The Ai14-tdTf/f reporter is a fluorescent reporter construct used in biomedical research. It contains a tandem dimer Tomato (tdTf) fluorescent protein expression cassette flanked by the attB sites for recombination-based gene insertion.

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Lab products found in correlation

B6 cg tg thy1 yfp hjrs j, by Jackson ImmunoResearch (2 mentions) Wt c57b 6j, by Jackson ImmunoResearch (2 mentions) Apoe ko b6.129p2 apoe tm1unc j, by Jackson ImmunoResearch (2 mentions) B6 c tg cmv cre 1cgn j, by Jackson ImmunoResearch (2 mentions) Tg slc1a3 cre ert 1nat j, by Jackson ImmunoResearch (1 mentions)

2 protocols using ai14 tdtf f reporter

1

Generation and Maintenance of Transgenic Mice

Cited 2 times
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CD63-GFP floxed mice were generated in the lab by homologous recombination, as previously described 35 (link). The WT (C57B/6J, #000664), Ai14-tdTf/f reporter (#007914), ApoE-KO (B6.129P2-Apoe tm1Unc/J #002052), B6.Cg-Tg (Thy1-YFP)HJrs/J (#003782), and B6.C-Tg(CMV-Cre)1Cgn/J(#006054) mice were obtained from the Jackson Laboratory. HepaCAM knock-out (KO) mice were generated by breeding HepaCAM floxed mice (a kind gift from Dr. Cagla Eroglu at Duke University)19 (link) with CMV-Cre mice. Both male and female mice were used in all experiments. All mice were maintained on a 12 h light/dark cycle with food and water ad libitum. Care and treatment of animals in all procedures strictly followed the NIH Guide for the Care and Use of Laboratory Animals and the Guidelines for the Use of Animals in Neuroscience Research. Animal protocols used in this study have been approved by the Tufts University IACUC.
Jin S., Chen X., Tian Y., Jarvis R., Promes V, & Yang Y. (2023). Astroglial exosome HepaCAM signaling and ApoE antagonization coordinates early postnatal cortical pyramidal neuronal axon growth and dendritic spine formation. bioRxiv.
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2

Generating Transgenic Mouse Models

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
hCD63-GFP floxed mice were generated in the lab by homologous recombination, as previously described35 (link). The WT (C57B/6J, #000664), Ai14-tdTf/f reporter (#007914), Tg(Slc1a3-cre/ERT)1Nat/J (#012586), ApoE-KO (B6.129P2-Apoe tm1Unc/J #002052), B6.Cg-Tg (Thy1-YFP)HJrs/J (#003782), and B6.C-Tg(CMV-Cre)1Cgn/J(#006054) mice were obtained from the Jackson Laboratory. HepaCAM knock-out (KO) mice were generated by breeding HepaCAM floxed mice (a kind gift from Dr. Cagla Eroglu at Duke University)19 (link) with CMV-Cre mice. Both male and female mice were used in all experiments. Both sexes were used in all studies. All mice were maintained on a 12 h light/dark cycle with food and water ad libitum.
Jin S., Chen X., Tian Y., Jarvis R., Promes V, & Yang Y. (2023). Astroglial exosome HepaCAM signaling and ApoE antagonization coordinates early postnatal cortical pyramidal neuronal axon growth and dendritic spine formation. Nature Communications, 14, 5150.
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