Human lymphocyte characterization was performed on an LSR II (BD Biosciences). Lungs from BLT mice were minced using scissors and incubated in medium containing Liberase (0.02 mg/ml) and DNase (5 mg/ml) (both from Sigma-Aldrich). Spleen, liver, thymus and lymph nodes were minced using scissors. Processed tissues were passed through a 40-μm mesh strainer to obtain single-cell suspensions. Remaining red blood cells were removed using RBC lysis buffer (Sigma-Aldrich). Cells were stained using the anti-human monoclonal antibodies fluorophore-conjugated (Biolegend) Brilliant Violet 605 anti-human CD45 (HI30), and PE/Cy7 anti-human CD4 Antibody (clone RPA-T4). Dead cells were excluded using LIVE/DEAD Fixable Blue Dead Cell Stain Kit (Life Technologies). Cells were fixed with 2% paraformaldehyde after staining. Whole blood (100 μL) was directly stained with antibodies and treated with FACS lysing solution (BD Biosciences) before analysis. CountBright Absolute Counting Beads (ThermoFisher) were used to determine cell numbers.
Brilliant violet 605 anti human cd45 hi30
Manufactured by BioLegend
Brilliant Violet 605 anti-human CD45 (HI30) is a fluorochrome-conjugated monoclonal antibody that binds to the CD45 cell surface antigen expressed on human leukocytes. It is designed for use in flow cytometry applications.
Automatically generated - may contain errors
Lab products found in correlation
Live dead fixable blue dead cell stain kit, by Thermo Fisher Scientific (2 mentions)
Facs lysing solution, by BD (2 mentions)
Dnase, by Merck Group (2 mentions)
Countbright absolute counting beads, by Thermo Fisher Scientific (2 mentions)
Liberase, by Merck Group (2 mentions)
Rbc lysis buffer, by Merck Group (2 mentions)
Pe cy7 anti human cd4 antibody, by BioLegend (2 mentions)
2 protocols using brilliant violet 605 anti human cd45 hi30
1
Comprehensive Immune Cell Profiling of BLT Mice
2
Comprehensive Immune Cell Profiling of BLT Mice
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Human lymphocyte characterization was performed on an LSR II (BD Biosciences). Lungs from BLT mice were minced using scissors and incubated in medium containing Liberase (0.02 mg/ml) and DNase (5 mg/ml) (both from Sigma-Aldrich). Spleen, liver, thymus and lymph nodes were minced using scissors. Processed tissues were passed through a 40-μm mesh strainer to obtain single-cell suspensions. Remaining red blood cells were removed using RBC lysis buffer (Sigma-Aldrich). Cells were stained using the anti-human monoclonal antibodies fluorophore-conjugated (Biolegend) Brilliant Violet 605 anti-human CD45 (HI30), and PE/Cy7 anti-human CD4 Antibody (clone RPA-T4). Dead cells were excluded using LIVE/DEAD Fixable Blue Dead Cell Stain Kit (Life Technologies). Cells were fixed with 2% paraformaldehyde after staining. Whole blood (100 μL) was directly stained with antibodies and treated with FACS lysing solution (BD Biosciences) before analysis. CountBright Absolute Counting Beads (ThermoFisher) were used to determine cell numbers.
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