Confocal laser scanning microscopy was performed using an Olympus FluoView FV1000 inverted confocal microscope. The 457-nm line of an argon laser was used to excite CFP and chlorophyll and the 514-nm line was used to excite YFP. The detection filters were set at 482–435 nm for CFP and 505–530 nm for YFP emission. Images were obtained and processed using software FV10-ASW (Ver4.1) (Olympus).
Fv10 asw ver4.1
Manufactured by Olympus
Sourced in Japan
The FV10-ASW (Ver4.1) is a software application for Olympus confocal microscopes. It provides image acquisition, analysis, and processing capabilities for users.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using fv10 asw ver4.1
1
Confocal Microscopy for Dual-Labeling
2
Endoxifen and Curcumin Effects on Cells
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Coverslip was coated using a poly-L-lysine. Coverslips were placed in each well of a six well cell culture plate. Cells (104) were seeded into each well of a 6-well plate. After 72-hour incubation to allow cells to adhere, cells were treated with endoxifen+β-estradiol with or without curcumin or DMSO every 48 hours until the 60% of cell confluency. Coverslips were washed and mounted on glass slide. All images were captured on Olympus Fluoview FV1,200 Confocal Laser Scanning Microscope (Olympus, Japan) using a 40× lens. Images were captured using FV10-ASW ver 4.1 and Cellsens ver 1.14 imaging software (Olympus, Japan).
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