Frozen tissue slices were mixed with beta-mercaptoethanol containing sample buffer and homogenized using the BeadBug system. DNA and RNA were extracted in parallel from the same sample using the Qiagen
AllPrep Universal Kit according to the manufacturer’s instructions.
DNA and RNA concentration were quantified using Qubit fluorometer with the Qubit dsDNA BR assay or Qubit RNA BR assay respectively.
DNA and RNA quality were assessed using the Agilent
Tapestation with
the Agilent Genomic DNA kit or Agilent
High-Sensitivity RNA ScreenTape kit respectively. RNAs need to have a RIN ≥ 4 or a DV200 ≥ 60 to be selected for library preparation.
Han Y., Rovella V., Smirnov A., Buonomo O.C., Mauriello A., Perretta T., Shi Y., Woodmsith J., Bischof J., Melino G., Candi E, & Bernassola F. (2023). A BRCA2 germline mutation and high expression of immune checkpoints in a TNBC patient. Cell Death Discovery, 9, 370.